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PMID:21695274

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Citation

Dixit, AR and Dhankher, OP (2011) A novel stress-associated protein 'AtSAP10' from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress. PLoS ONE 6:e20921

Abstract

We describe here the functional characterization of a novel AtSAP10, a member of the Stress Associated Protein (SAP) gene family, from Arabidopsis thaliana ecotype Columbia. AtSAP10 contains an A20 and AN1 zinc-finger domain at the N- and C-terminal, respectively. Arabidopsis SAP10 showed differential regulation by various abiotic stresses such as heavy metals and metalloids (Ni, Cd, Mn, Zn, and As), high and low temperatures, cold, and ABA. Overexpression of AtSAP10 in Arabidopsis conferred strong tolerance to heavy metals such as Ni, Mn, and Zn and to high temperature stress. AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants. Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn. AtSAP10 promoter-GUS fusion studies revealed a root and floral organ-specific expression of AtSAP10. Overexpression of AtSAP10-GFP fusion protein showed the localization in both nucleus and cytoplasm. Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

Links

PubMed PMC3111467 Online version:10.1371/journal.pone.0020921

Keywords

Arabidopsis/cytology; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DNA, Bacterial/genetics; Gene Expression Regulation, Plant/drug effects; Heat-Shock Response/genetics; Intracellular Space/drug effects; Intracellular Space/metabolism; Manganese/metabolism; Manganese/toxicity; Metals, Heavy/metabolism; Metals, Heavy/toxicity; Nickel/metabolism; Nickel/toxicity; Protein Transport/drug effects; Zinc/metabolism; Zinc/toxicity; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SAP10

acts_upstream_of_or_within

GO:0034605: cellular response to heat

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SAP10

acts_upstream_of_or_within

GO:0010045: response to nickel cation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SAP10

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SAP10

acts_upstream_of_or_within

GO:0010042: response to manganese ion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SAP10

acts_upstream_of_or_within

GO:0010038: response to metal ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SAP10

acts_upstream_of_or_within

GO:0071472: cellular response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SAP10

acts_upstream_of_or_within

GO:0070417: cellular response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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