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PMID:21690391

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Citation

Krichevsky, A, Zaltsman, A, Lacroix, B and Citovsky, V (2011) Involvement of KDM1C histone demethylase-OTLD1 otubain-like histone deubiquitinase complexes in plant gene repression. Proc. Natl. Acad. Sci. U.S.A. 108:11157-62

Abstract

Covalent modifications of histones, such as acetylation, methylation and ubiquitination, are central for regulation of gene expression. Heterochromatic gene silencing, for example, is associated with hypoacetylation, methylation and demethylation, and deubiquitination of specific amino acid residues in histone molecules. Many of these changes can be effected by histone-modifying repressor complexes that include histone lysine demethylases, such as KDM1 in animals and KDM1C in plants. However, whereas KDM1-containing repressor complexes have been implicated in histone demethylation, methylation and deacetylation, whether or not they can also mediate histone deubiquitination remains unknown. We identify an Arabidopsis otubain-like deubiquitinase OTLD1 which directly interacts with the Arabidopsis KDM1C in planta, and use one target gene to exemplify that both OTLD1 and KDM1C are involved in transcriptional gene repression via histone deubiquitination and demethylation. We also show that OTLD1 binds plant chromatin and has enzymatic histone deubiquitinase activity, specific for the H2B histone. Thus, we suggest that, during gene repression, lysine demethylases can directly interact and function in a protein complex with histone deubiquitinases.

Links

PubMed PMC3131378 Online version:10.1073/pnas.1014030108

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Chromatin/genetics; Chromatin/metabolism; Cysteine Proteases/genetics; Cysteine Proteases/metabolism; DNA Primers/genetics; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Histone Demethylases/genetics; Histone Demethylases/metabolism; Histones/chemistry; Histones/metabolism; Molecular Sequence Data; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Plants, Genetically Modified; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sequence Homology, Amino Acid; Two-Hybrid System Techniques; Ubiquitination

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:LDL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9XIP2

F

Seeded From UniProt

complete

ARATH:LDL1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:LDL1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:LDL1

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:OTU6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8VXV7

F

Seeded From UniProt

complete

ARATH:OTU6

involved_in

GO:0016578: histone deubiquitination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:OTU6

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:OTU6

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:OTU6

enables

GO:0031491: nucleosome binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:OTU6

enables

GO:0042393: histone binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:OTU6

enables

GO:0003682: chromatin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:OTU6

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:OTU6

acts_upstream_of_or_within

GO:0016578: histone deubiquitination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:OTU6

acts_upstream_of_or_within

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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