GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:21658587

From GONUTS
Jump to: navigation, search
Citation

Erskine, L, Reijntjes, S, Pratt, T, Denti, L, Schwarz, Q, Vieira, JM, Alakakone, B, Shewan, D and Ruhrberg, C (2011) VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. Neuron 70:951-65

Abstract

During development, the axons of retinal ganglion cell (RGC) neurons must decide whether to cross or avoid the midline at the optic chiasm to project to targets on both sides of the brain. By combining genetic analyses with in vitro assays, we show that neuropilin 1 (NRP1) promotes contralateral RGC projection in mammals. Unexpectedly, the NRP1 ligand involved is not an axon guidance cue of the class 3 semaphorin family, but VEGF164, the neuropilin-binding isoform of the classical vascular growth factor VEGF-A. VEGF164 is expressed at the chiasm midline and is required for normal contralateral growth in vivo. In outgrowth and growth cone turning assays, VEGF164 acts directly on NRP1-expressing contralateral RGCs to provide growth-promoting and chemoattractive signals. These findings have identified a permissive midline signal for axons at the chiasm midline and provide in vivo evidence that VEGF-A is an essential axon guidance cue.

Links

PubMed PMC3114076 Online version:10.1016/j.neuron.2011.02.052

Keywords

Age Factors; Amino Acids/metabolism; Animals; Axons/drug effects; Embryo, Mammalian; Functional Laterality; Gene Expression Regulation, Developmental/genetics; Gene Expression Regulation, Developmental/physiology; Growth Cones/physiology; Indoles/metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Neuropilin-1/deficiency; Neuropilin-1/genetics; Neuropilin-1/metabolism; Neuropilin-2/deficiency; Optic Chiasm/drug effects; Optic Chiasm/embryology; Optic Chiasm/physiology; Organ Culture Techniques; Retina/cytology; Retina/drug effects; Retina/embryology; Retinal Ganglion Cells/cytology; Retinal Ganglion Cells/drug effects; Retinal Ganglion Cells/physiology; Semaphorin-3A/deficiency; Signal Transduction/genetics; Signal Transduction/physiology; Vascular Endothelial Growth Factor A/genetics; Vascular Endothelial Growth Factor A/pharmacology; Vascular Endothelial Growth Factor Receptor-1/genetics; Vascular Endothelial Growth Factor Receptor-2/genetics; Vascular Endothelial Growth Factors/genetics; Vascular Endothelial Growth Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NRP1

located_in

GO:0030424: axon

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000740)
  • part_of:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:NRP1

enables

GO:0005021: vascular endothelial growth factor-activated receptor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

  • occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:0050918: positive chemotaxis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:1902378: VEGF-activated neuropilin signaling pathway involved in axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0050918: positive chemotaxis

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:VEGFA

enables

GO:0042056: chemoattractant activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:VEGFA

NOT|involved_in

GO:0048842: positive regulation of axon extension involved in axon guidance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:VEGFA

NOT|involved_in

GO:0071679: commissural neuron axon guidance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:VEGFA

NOT|involved_in

GO:0090259: regulation of retinal ganglion cell axon guidance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0038190: VEGF-activated neuropilin signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • causally_upstream_of:(GO:1902336)

Seeded From UniProt

complete

MOUSE:VEGFA

enables

GO:0042056: chemoattractant activity

ECO:0000314: direct assay evidence used in manual assertion

F

occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0048842: positive regulation of axon extension involved in axon guidance

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0071679: commissural neuron axon guidance

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0090259: regulation of retinal ganglion cell axon guidance

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:1902336: positive regulation of retinal ganglion cell axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0048842: positive regulation of axon extension involved in axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0050918: positive chemotaxis

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0071679: commissural neuron axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:VEGFA

involved_in

GO:0090259: regulation of retinal ganglion cell axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:1902378: VEGF-activated neuropilin signaling pathway involved in axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:1902336: positive regulation of retinal ganglion cell axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:0090259: regulation of retinal ganglion cell axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:0071679: commissural neuron axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:0050918: positive chemotaxis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

occurs_in:(CL:0000740)

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:0048842: positive regulation of axon extension involved in axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000740)
  • occurs_in:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:NRP1

involved_in

GO:0031290: retinal ganglion cell axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NRP1

part_of

GO:0030424: axon

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000740)
  • part_of:(UBERON:0000959)

Seeded From UniProt

complete

MOUSE:NRP1

enables

GO:0005021: vascular endothelial growth factor-activated receptor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

occurs_in:(CL:0000740)

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.