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PMID:21623367

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Citation

Becker, T, Armache, JP, Jarasch, A, Anger, AM, Villa, E, Sieber, H, Motaal, BA, Mielke, T, Berninghausen, O and Beckmann, R (2011) Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome. Nat. Struct. Mol. Biol. 18:715-20

Abstract

No-go decay (NGD) is a mRNA quality-control mechanism in eukaryotic cells that leads to degradation of mRNAs stalled during translational elongation. The key factors triggering NGD are Dom34 and Hbs1. We used cryo-EM to visualize NGD intermediates resulting from binding of the Dom34-Hbs1 complex to stalled ribosomes. At subnanometer resolution, all domains of Dom34 and Hbs1 were identified, allowing the docking of crystal structures and homology models. Moreover, the close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled us to propose a model for the ribosome-bound eRF1-eRF3 complex. Collectively, our data provide structural insights into how stalled mRNA is recognized on the ribosome and how the eRF complex can simultaneously recognize stop codons and catalyze peptide release.

Links

PubMed Online version:10.1038/nsmb.2057

Keywords

Cell Cycle Proteins/chemistry; Cell Cycle Proteins/metabolism; Cell Cycle Proteins/ultrastructure; Cryoelectron Microscopy; Endoribonucleases/chemistry; Endoribonucleases/metabolism; Endoribonucleases/ultrastructure; GTP-Binding Proteins/chemistry; GTP-Binding Proteins/metabolism; GTP-Binding Proteins/ultrastructure; HSP70 Heat-Shock Proteins/chemistry; HSP70 Heat-Shock Proteins/metabolism; HSP70 Heat-Shock Proteins/ultrastructure; Models, Molecular; Peptide Elongation Factors/chemistry; Peptide Elongation Factors/metabolism; Peptide Elongation Factors/ultrastructure; Protein Binding; RNA Stability; RNA, Messenger/chemistry; RNA, Messenger/metabolism; Ribosomes/chemistry; Ribosomes/metabolism; Ribosomes/ultrastructure; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:HBS1

part_of

GO:1990533: Dom34-Hbs1 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:HBS1

contributes_to

GO:0043022: ribosome binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:HBS1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P33309

F

Seeded From UniProt

complete

YEAST:DOM34

part_of

GO:1990533: Dom34-Hbs1 complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:DOM34

contributes_to

GO:0043022: ribosome binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:DOM34

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P32769

F

Seeded From UniProt

complete


See also

References

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