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PMID:2161540

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Citation

Mansukhani, A, Moscatelli, D, Talarico, D, Levytska, V and Basilico, C (1990) A murine fibroblast growth factor (FGF) receptor expressed in CHO cells is activated by basic FGF and Kaposi FGF. Proc. Natl. Acad. Sci. U.S.A. 87:4378-82

Abstract

We have cloned a murine cDNA encoding a tyrosine kinase receptor with about 90% similarity to the chicken fibroblast growth factor (FGF) receptor and the human fms-like gene (FLG) tyrosine kinase. This mouse receptor lacks 88 amino acids in the extracellular portion, leaving only two immunoglobulin-like domains compared to three in the chicken FGF receptor. The cDNA was cloned into an expression vector and transfected into receptor-negative CHO cells. We show that cells expressing the receptor can bind both basic FGF and Kaposi FGF. Although the receptor binds basic FGF with a 15- to 20-fold higher affinity, Kaposi FGF is able to induce down-regulation of the receptor to the same extent as basic FGF. The receptor is phosphorylated upon stimulation with both FGFs, DNA synthesis is stimulated, and a proliferative response is produced in cells expressing the receptor, whereas cells expressing the cDNA in the antisense orientation show none of these responses to basic FGF or Kaposi FGF. Thus this receptor can functionally interact with two growth factors of the FGF family.

Links

PubMed PMC54113

Keywords

Amino Acid Sequence; Animals; Blotting, Western; Cell Division; Cell Line; Cloning, Molecular; Cricetinae; Cricetulus; DNA/genetics; Down-Regulation; Fibroblast Growth Factors/physiology; Mice; Molecular Sequence Data; Phosphorylation; Protein-Tyrosine Kinases/physiology; Receptors, Cell Surface/physiology; Receptors, Fibroblast Growth Factor; Sarcoma, Kaposi/physiopathology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FGFR1

acts_upstream_of_or_within

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P09038

P

Seeded From UniProt

complete

MOUSE:FGFR1

acts_upstream_of_or_within

GO:0008284: positive regulation of cell population proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:FGFR1

enables

GO:0017134: fibroblast growth factor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:FGFR1

enables

GO:0005007: fibroblast growth factor-activated receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:FGFR1

located_in

GO:0005886: plasma membrane

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P09038

C

Seeded From UniProt

complete

MOUSE:FGFR1

acts_upstream_of_or_within

GO:1903465: positive regulation of mitotic cell cycle DNA replication

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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