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PMID:21575913

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Citation

Dierking, K, Polanowska, J, Omi, S, Engelmann, I, Gut, M, Lembo, F, Ewbank, JJ and Pujol, N (2011) Unusual regulation of a STAT protein by an SLC6 family transporter in C. elegans epidermal innate immunity. Cell Host Microbe 9:425-35

Abstract

The cuticle and epidermis of Caenorhabditis elegans provide the first line of defense against invading pathogens. Upon invasion by the fungal pathogen Drechmeria coniospora, C. elegans responds by upregulating the expression of antimicrobial peptides (AMPs) in the epidermis via activation of at least two pathways, a neuroendocrine TGF-β pathway and a p38 MAPK pathway. Here, we identify the sodium-neurotransmitter symporter SNF-12, a member of the solute carrier family (SLC6), as being essential for both these immune signaling pathways. We also identify the STAT transcription factor-like protein STA-2 as a direct physical interactor of SNF-12 and show that the two proteins function together to regulate AMP gene expression in the epidermis. Both SNF-12 and STA-2 act cell autonomously and specifically in the epidermis to govern the transcriptional response to fungal infection. These findings reveal an unorthodox mode of regulation for a STAT factor and highlight the molecular plasticity of innate immune signaling.

Links

PubMed Online version:10.1016/j.chom.2011.04.011

Keywords

Animals; Antimicrobial Cationic Peptides/biosynthesis; Caenorhabditis elegans/genetics; Caenorhabditis elegans/immunology; Caenorhabditis elegans Proteins/metabolism; Epidermis/immunology; GABA Plasma Membrane Transport Proteins/metabolism; Gene Expression Regulation; Hypocreales/immunology; Immunity, Innate; Models, Biological; Protein Binding; Protein Interaction Mapping; STAT Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:O45813

enables

GO:0008134: transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00010251

F

Seeded From UniProt

complete

CAEEL:O45813

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:O45813

involved_in

GO:0002804: positive regulation of antifungal peptide production

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00145343
WB:WBVar00249722

P

Seeded From UniProt

complete

CAEEL:O45813

located_in

GO:0030139: endocytic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CLH

located_in

GO:0030139: endocytic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:STATB

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:STATB

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:STATB

involved_in

GO:0002804: positive regulation of antifungal peptide production

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00093057

P

Seeded From UniProt

complete

CAEEL:STATB

located_in

GO:0030139: endocytic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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