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PMID:2155780

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Citation

Grenningloh, G, Schmieden, V, Schofield, PR, Seeburg, PH, Siddique, T, Mohandas, TK, Becker, CM and Betz, H (1990) Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes. EMBO J. 9:771-6

Abstract

Two cDNAs encoding variants (alpha 1 and alpha 2) of the strychnine binding subunit of the inhibitory glycine receptor (GlyR) were isolated from a human fetal brain cDNA library. The predicted amino acid sequences exhibit approximately 99% and approximately 76% identity to the previously characterized rat 48 kd polypeptide. Heterologous expression of the human alpha 1 and alpha 2 subunits in Xenopus oocytes resulted in the formation of glycine-gated strychnine-sensitive chloride channels, indicating that both polypeptides can form functional GlyRs. Using a panel of rodent-human hybrid cell lines, the gene encoding alpha 2 was mapped to the short arm (Xp21.2-p22.1) of the human X chromosome. In contrast, the alpha 1 subunit gene is autosomally located. These data indicate molecular heterogeneity of the human GlyR at the level of alpha subunit genes.

Links

PubMed PMC551735

Keywords

Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; Chromosome Mapping; Cloning, Molecular; DNA/genetics; Gene Expression; Genetic Variation; Glycine/metabolism; Humans; Hybrid Cells/metabolism; Macromolecular Substances; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Rats; Receptors, Glycine; Receptors, Neurotransmitter/genetics; Sequence Homology, Nucleic Acid; X Chromosome; Xenopus

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:GLRA1

located_in

GO:0005887: integral component of plasma membrane

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

C

Seeded From UniProt

complete

RAT:GLRA1

located_in

GO:0043231: intracellular membrane-bounded organelle

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

C

Seeded From UniProt

complete

HUMAN:GLRA1

located_in

GO:0005887: integral component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:GLRA1

involved_in

GO:0007218: neuropeptide signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLRA1

involved_in

GO:0006811: ion transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLRA1

enables

GO:0016594: glycine binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:GLRA1

enables

GO:0016934: extracellularly glycine-gated chloride channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:GLRA2

located_in

GO:0005887: integral component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:GLRA2

involved_in

GO:0034220: ion transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLRA2

involved_in

GO:0071230: cellular response to amino acid stimulus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLRA2

involved_in

GO:0007218: neuropeptide signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLRA2

enables

GO:0016594: glycine binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:GLRA2

enables

GO:0016934: extracellularly glycine-gated chloride channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:GLRA1

involved_in

GO:0001964: startle response

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

P

Seeded From UniProt

complete

RAT:GLRA1

part_of

GO:0005887: integral component of plasma membrane

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

C

Seeded From UniProt

complete

RAT:GLRA1

involved_in

GO:0006811: ion transport

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

P

Seeded From UniProt

complete

RAT:GLRA1

involved_in

GO:0006821: chloride transport

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

P

Seeded From UniProt

complete

RAT:GLRA1

involved_in

GO:0006936: muscle contraction

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

P

Seeded From UniProt

complete

RAT:GLRA1

involved_in

GO:0007218: neuropeptide signaling pathway

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

P

Seeded From UniProt

complete

RAT:GLRA1

enables

GO:0016594: glycine binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

F

Seeded From UniProt

complete

RAT:GLRA1

enables

GO:0016934: extracellularly glycine-gated chloride channel activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

F

Seeded From UniProt

complete

RAT:GLRA1

enables

GO:0030977: taurine binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

F

Seeded From UniProt

complete

RAT:GLRA1

part_of

GO:0043231: intracellular membrane-bounded organelle

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

C

Seeded From UniProt

complete

RAT:GLRA1

involved_in

GO:0051970: negative regulation of transmission of nerve impulse

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P23415

P

Seeded From UniProt

complete

Notes

See also

References

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