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PMID:21531871

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Citation

Pribic, J, Garcia, R, Kong, M and Brazill, D (2011) Paxillin and phospholipase D interact to regulate actin-based processes in Dictyostelium discoideum. Eukaryotic Cell 10:977-84

Abstract

The actin cytoskeleton forms a membrane-associated network whose proper regulation is essential for numerous processes, including cell differentiation, proliferation, adhesion, chemotaxis, endocytosis, exocytosis, and multicellular development. In this report, we show that in Dictyostelium discoideum, paxillin (PaxB) and phospholipase D (PldB) colocalize and coimmunoprecipitate, suggesting that they interact physically. Additionally, the phenotypes observed during development, cell sorting, and several actin-required processes, including cyclic AMP (cAMP) chemotaxis, cell-substrate adhesion, actin polymerization, phagocytosis, and exocytosis, reveal a genetic interaction between paxB and pldB, suggesting a functional interaction between their gene products. Taken together, our data point to PldB being a required binding partner of PaxB during processes involving actin reorganization.

Links

PubMed PMC3147424 Online version:10.1128/EC.00282-10

Keywords

Actins/metabolism; Cell Adhesion; Cell Differentiation; Cell Movement; Cell Proliferation; Cyclic AMP/metabolism; Cytoskeleton/metabolism; Dictyostelium/cytology; Dictyostelium/genetics; Dictyostelium/metabolism; Endocytosis/physiology; Exocytosis/physiology; Gene Knockout Techniques; Immunoprecipitation; Paxillin/genetics; Paxillin/metabolism; Phagocytosis/physiology; Phospholipase D/genetics; Phospholipase D/metabolism; Protozoan Proteins/genetics; Protozoan Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:PLDB

part_of

GO:0031941: filamentous actin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:PLDB

part_of

GO:0032991: protein-containing complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:PLDB

NOT|acts_upstream_of_or_within

GO:0043326: chemotaxis to folate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PLDB

acts_upstream_of_or_within

GO:0043327: chemotaxis to cAMP

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PLDB

acts_upstream_of_or_within

GO:0045921: positive regulation of exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PLDB

acts_upstream_of_or_within

GO:2000249: regulation of actin cytoskeleton reorganization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:2000249: regulation of actin cytoskeleton reorganization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0050765: negative regulation of phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

part_of

GO:0032991: protein-containing complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:PAXB

part_of

GO:0031941: filamentous actin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:PAXB

NOT|acts_upstream_of_or_within

GO:0043326: chemotaxis to folate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0043327: chemotaxis to cAMP

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PAXB

acts_upstream_of_or_within

GO:0045921: positive regulation of exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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