PMID:21529875

Citation	Brindley, MA, Hunt, CL, Kondratowicz, AS, Bowman, J, Sinn, PL, McCray, PB Jr, Quinn, K, Weller, ML, Chiorini, JA and Maury, W (2011) Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein. Virology 415:83-94
Abstract	In a bioinformatics-based screen for cellular genes that enhance Zaire ebolavirus (ZEBOV) transduction, AXL mRNA expression strongly correlated with ZEBOV infection. A series of cell lines and primary cells were identified that require Axl for optimal ZEBOV entry. Using one of these cell lines, we identified ZEBOV entry events that are Axl-dependent. Interactions between ZEBOV-GP and the Axl ectodomain were not detected in immunoprecipitations and reduction of surface-expressed Axl by RNAi did not alter ZEBOV-GP binding, providing evidence that Axl does not serve as a receptor for the virus. However, RNAi knock down of Axl reduced ZEBOV pseudovirion internalization and α-Axl antisera inhibited pseudovirion fusion with cellular membranes. Consistent with the importance of Axl for ZEBOV transduction, Axl transiently co-localized on the surface of cells with ZEBOV virus particles and was internalized during virion transduction. In total, these findings indicate that endosomal uptake of filoviruses is facilitated by Axl.
Links	PubMed PMC3107944 Online version:10.1016/j.virol.2011.04.002
Keywords	Cell Line, Tumor; Ebolavirus/genetics; Ebolavirus/physiology; Glycoproteins; Hemorrhagic Fever, Ebola/enzymology; Hemorrhagic Fever, Ebola/genetics; Hemorrhagic Fever, Ebola/metabolism; Hemorrhagic Fever, Ebola/virology; Humans; Protein Binding; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; Receptor Protein-Tyrosine Kinases/genetics; Receptor Protein-Tyrosine Kinases/metabolism; Viral Envelope Proteins/genetics; Viral Envelope Proteins/metabolism; Virus Internalization
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:UFO	part_of	GO:0044228: host cell surface	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
HUMAN:UFO		GO:0044228: host cell surface	ECO:0000314:		C		Fig. 7 and Fig. 8	complete CACAO 2202

References

See Help:References for how to manage references in GONUTS.