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PMID:21515584

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Citation

Both, P, Sobczak, L, Breton, C, Hann, S, Nöbauer, K, Paschinger, K, Kozmon, S, Mucha, J and Wilson, IB (2011) Distantly related plant and nematode core α1,3-fucosyltransferases display similar trends in structure-function relationships. Glycobiology 21:1401-15

Abstract

Here, we present a comparative structure-function study of a nematode and a plant core α1,3-fucosyltransferase based on deletion and point mutations of the coding regions of Caenorhabditis elegans FUT-1 and Arabidopsis thaliana FucTA (FUT11). In particular, our results reveal a novel "first cluster motif" shared by both core and Lewis-type α1,3-fucosyltransferases of the GT10 family. To evaluate the role of the conserved serine within this motif, this residue was replaced with alanine in FucTA (S218) and FUT-1 (S243). The S218A replacement completely abolished the enzyme activity of FucTA, while the S243A mutant of FUT-1 retained 20% of the "wild-type" activity. Based on the results of homology modeling of FucTA, other residues potentially involved in the donor substrate binding were examined, and mutations of N219 and R226 dramatically affected enzymatic activity. Finally, as both FucTA and FUT-1 were shown to be N-glycosylated, we examined the putative N-glycosylation sites. While alanine replacements at single potential N-glycosylation sites of FucTA resulted in a loss of up to 80% of the activity, a triple glycosylation site mutant still retained 5%, as compared to the control. In summary, our data indicate similar trends in structure-function relationships of distantly related enzymes which perform similar biochemical reactions and form the basis for future work aimed at understanding the structure of α1,3-fucosyltransferases in general.

Links

PubMed PMC3272405 Online version:10.1093/glycob/cwr056

Keywords

Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution; Animals; Arabidopsis/enzymology; Binding Sites; Caenorhabditis elegans/enzymology; Cations, Divalent; Conserved Sequence; Enzyme Assays; Fucosyltransferases/biosynthesis; Fucosyltransferases/chemistry; Glycosylation; Magnesium; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments/chemistry; Protein Structure, Tertiary; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Structural Homology, Protein; Tandem Mass Spectrometry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:FUT11

enables

GO:0046920: alpha-(1->3)-fucosyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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