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PMID:21494665

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Citation

Sun, SC, Wang, ZB, Xu, YN, Lee, SE, Cui, XS and Kim, NH (2011) Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes. PLoS ONE 6:e18392

Abstract

Mammalian oocyte meiotic maturation involves oocyte polarization and a unique asymmetric division, but until now, the underlying mechanisms have been poorly understood. Arp2/3 complex has been shown to regulate actin nucleation and is widely involved in a diverse range of processes such as cell locomotion, phagocytosis and the establishment of cell polarity. Whether Arp2/3 complex participates in oocyte polarization and asymmetric division is unknown. The present study investigated the expression and functions of Arp2/3 complex during mouse oocyte meiotic maturation. Immunofluorescent staining showed that the Arp2/3 complex was restricted to the cortex, with a thickened cap above the meiotic apparatus, and that this localization pattern was depended on actin. Disruption of Arp2/3 complex by a newly-found specific inhibitor CK666, as well as by Arpc2 and Arpc3 RNAi, resulted in a range of effects. These included the failure of asymmetric division, spindle migration, and the formation and completion of oocyte cytokinesis. The formation of the actin cap and cortical granule-free domain (CGFD) was also disrupted, which further confirmed the disruption of spindle migration. Our data suggest that the Arp2/3 complex probably regulates oocyte polarization through its effect on spindle migration, asymmetric division and cytokinesis during mouse oocyte meiotic maturation.

Links

PubMed PMC3072972 Online version:10.1371/journal.pone.0018392

Keywords

Actin-Related Protein 2-3 Complex/metabolism; Actins/metabolism; Animals; Cell Differentiation/drug effects; Cell Polarity/drug effects; Cytochalasin B/pharmacology; Cytokinesis/drug effects; Cytoplasmic Granules/drug effects; Cytoplasmic Granules/metabolism; Indoles/pharmacology; Meiosis/drug effects; Mice; Mitotic Spindle Apparatus/drug effects; Mitotic Spindle Apparatus/metabolism; Models, Biological; Oocytes/cytology; Oocytes/drug effects; Oocytes/metabolism; Protein Transport/drug effects; RNA Interference/drug effects

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ARP2

acts_upstream_of_or_within

GO:0033206: meiotic cytokinesis

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1921367

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

located_in

GO:0030478: actin cap

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

acts_upstream_of_or_within

GO:0007163: establishment or maintenance of cell polarity

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1921367

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

acts_upstream_of_or_within

GO:0008356: asymmetric cell division

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1921367

P

Seeded From UniProt

complete

MOUSE:ARP2

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

acts_upstream_of_or_within

GO:0051653: spindle localization

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1921367

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

acts_upstream_of_or_within

GO:0051321: meiotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1921367

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP3

acts_upstream_of_or_within

GO:0033206: meiotic cytokinesis

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1913963

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP3

acts_upstream_of_or_within

GO:0007163: establishment or maintenance of cell polarity

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1913963

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP3

acts_upstream_of_or_within

GO:0008356: asymmetric cell division

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1913963

P

Seeded From UniProt

complete

MOUSE:ARP3

acts_upstream_of_or_within

GO:0051653: spindle localization

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1913963

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP3

acts_upstream_of_or_within

GO:0051321: meiotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1913963

P

Seeded From UniProt

complete


See also

References

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