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PMID:21471120

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Citation

Kim, YY, Jung, KW, Yoo, KS, Jeung, JU and Shin, JS (2011) A stress-responsive caleosin-like protein, AtCLO4, acts as a negative regulator of ABA responses in Arabidopsis. Plant Cell Physiol. 52:874-84

Abstract

Caleosins or related sequences have been found in a wide range of higher plants. In Arabidopsis, seed-specific caleosins are viewed as oil-body (OB)-associated proteins that possess Ca(2+)-dependent peroxygenase activity and are involved in processes of lipid degradation. Recent experimental evidence suggests that one of the Arabidopsis non-seed caleosins, AtCLO3, is involved in controlling stomatal aperture during the drought response; the roles of the other caleosin-like proteins in Arabidopsis remain largely uncharacterized. We have demonstrated that a novel stress-responsive and OB-associated Ca(2+)-binding caleosin-like protein, AtCLO4, is expressed in non-seed tissues of Arabidopsis, including guard cells, and down-regulated following exposure to exogenous ABA and salt stress. At the seed germination stage, a loss-of-function mutant (atclo4) was hypersensitive to ABA, salt and mannitol stresses, whereas AtCLO4-overexpressing (Ox) lines were more hyposensitive to those stresses than the wild type. In adult stage, atclo4 mutant and AtCLO4-Ox plants showed enhanced and decreased drought tolerance, respectively. Following exposure to exogenous ABA, the expression of key ABA-dependent regulatory genes, such as ABF3 and ABF4, was up-regulated in the atclo4 mutant, while it was down-regulated in AtCLO4-Ox lines. Based on these results, we propose that the OB-associated Ca(2+)-binding AtCLO4 protein acts as a negative regulator of ABA responses in Arabidopsis.

Links

PubMed Online version:10.1093/pcp/pcr039

Keywords

Abscisic Acid/pharmacology; Adaptation, Physiological/drug effects; Arabidopsis/cytology; Arabidopsis/drug effects; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Calcium-Binding Proteins/metabolism; DNA, Bacterial/genetics; Droughts; Gene Expression Regulation, Plant/drug effects; Gene Knockdown Techniques; Genes, Plant/genetics; Germination/drug effects; Mannitol/pharmacology; Mutagenesis, Insertional/drug effects; Mutagenesis, Insertional/genetics; Mutation/genetics; Organ Specificity/drug effects; Plant Proteins/metabolism; Plant Stomata/cytology; Plant Stomata/drug effects; Plant Stomata/physiology; Plant Vascular Bundle/cytology; Plant Vascular Bundle/drug effects; Plant Vascular Bundle/metabolism; Plants, Genetically Modified; Promoter Regions, Genetic/genetics; Protein Binding/drug effects; Seeds/drug effects; Seeds/growth & development; Signal Transduction/genetics; Sodium Chloride/pharmacology; Stress, Physiological/drug effects; Subcellular Fractions/drug effects; Subcellular Fractions/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PXG4

acts_upstream_of_or_within

GO:0009819: drought recovery

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PXG4

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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