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PMID:21454751

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Citation

Oh, JS, Susor, A and Conti, M (2011) Protein tyrosine kinase Wee1B is essential for metaphase II exit in mouse oocytes. Science 332:462-5

Abstract

Waves of cyclin synthesis and degradation regulate the activity of Cdc2 protein kinase during the cell cycle. Cdc2 inactivation by Wee1B-mediated phosphorylation is necessary for arrest of the oocyte at G2-prophase, but it is unclear whether this regulation functions later during the metaphase-to-anaphase transition. We show that reactivation of a Wee1B pathway triggers the decrease in Cdc2 activity during egg activation. When Wee1B is down-regulated, oocytes fail to form a pronucleus in response to Ca(2+) signals. Calcium-calmodulin-dependent kinase II (CaMKII) activates Wee1B, and CaMKII-driven exit from metaphase II is inhibited by Wee1B down-regulation, demonstrating that exit from metaphase requires not only a proteolytic degradation of cyclin B but also the inhibitory phosphorylation of Cdc2 by Wee1B.

Links

PubMed Online version:10.1126/science.1199211

Keywords

Animals; CDC2 Protein Kinase/antagonists & inhibitors; CDC2 Protein Kinase/metabolism; Calcium/metabolism; Calcium Signaling; Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cyclin B/genetics; Cyclin B/metabolism; Down-Regulation; Female; Gene Knockdown Techniques; Maturation-Promoting Factor/metabolism; Meiosis; Metaphase; Mice; Mice, Inbred C57BL; Oocytes/physiology; Phosphorylation; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:WEE2

involved_in

GO:0035038: female pronucleus assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WEE2

enables

GO:0004713: protein tyrosine kinase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

MOUSE:WEE2

involved_in

GO:0045736: negative regulation of cyclin-dependent protein serine/threonine kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(UniProtKB:P11440)

Seeded From UniProt

complete

MOUSE:WEE2

involved_in

GO:1900194: negative regulation of oocyte maturation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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