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PMID:21447788

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Citation

Li, M, Bahn, SC, Guo, L, Musgrave, W, Berg, H, Welti, R and Wang, X (2011) Patatin-related phospholipase pPLAIIIβ-induced changes in lipid metabolism alter cellulose content and cell elongation in Arabidopsis. Plant Cell 23:1107-23

Abstract

The release of fatty acids from membrane lipids has been implicated in various plant processes, and the patatin-related phospholipases (pPLAs) constitute a major enzyme family that catalyzes fatty acid release. The Arabidopsis thaliana pPLA family has 10 members that are classified into three groups. Group 3 pPLAIII has four members but lacks the canonical lipase/esterase consensus catalytic sequences, and their enzymatic activity and cellular functions have not been delineated. Here, we show that pPLAIIIβ hydrolyzes phospholipids and galactolipids and additionally has acyl-CoA thioesterase activity. Alterations of pPLAIIIβ result in changes in lipid levels and composition. pPLAIIIβ-KO plants have longer leaves, petioles, hypocotyls, primary roots, and root hairs than wild-type plants, whereas pPLAIIIβ-OE plants exhibit the opposite phenotype. In addition, pPLAIIIβ-OE plants have significantly lower cellulose content and mechanical strength than wild-type plants. Root growth of pPLAIIIβ-KO plants is less sensitive to treatment with free fatty acids, the enzymatic products of pPLAIIIβ, than wild-type plants; root growth of pPLAIIIβ-OE plants is more sensitive. These data suggest that alteration of pPLAIIIβ expression and the resulting lipid changes alter cellulose content and cell elongation in Arabidopsis.

Links

PubMed PMC3082257 Online version:10.1105/tpc.110.081240

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Enlargement; Cell Membrane/metabolism; Cellulose/analysis; Fatty Acids, Nonesterified/analysis; Fatty Acids, Nonesterified/pharmacology; Gene Expression Regulation, Plant; Lipid Metabolism; Molecular Sequence Data; Phospholipases A/genetics; Phospholipases A/metabolism; Phospholipids/metabolism; Plant Epidermis/growth & development; Plant Leaves/growth & development; Plant Roots/enzymology; Plant Roots/growth & development; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PLP7

acts_upstream_of_or_within

GO:0019374: galactolipid metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLP7

acts_upstream_of_or_within

GO:0006644: phospholipid metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLP7

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PLP7

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PLP7

enables

GO:0047617: acyl-CoA hydrolase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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