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PMID:21441307

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Citation

Tower, RJ, Fagarasanu, A, Aitchison, JD and Rachubinski, RA (2011) The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast. Mol. Biol. Cell 22:1727-38

Abstract

Peroxisomes are ubiquitous organelles involved in diverse metabolic processes, most notably the metabolism of lipids and the detoxification of reactive oxygen species. Peroxisomes are highly dynamic and change in size and number in response to both intra- and extracellular cues. In the yeast Saccharomyces cerevisiae, peroxisome growth and division are controlled by both the differential import of soluble matrix proteins and a specialized divisional machinery that includes peroxisome-specific factors, such as members of the Pex11 protein family, and general organelle divisional factors, such as the dynamin-related protein Vps1p. Global yeast two-hybrid analyses have demonstrated interactions between the product of the S. cerevisiae gene of unknown function, YCL056c, and Pex proteins involved in peroxisome biogenesis. Here we show that the protein encoded by YCL056c, renamed Pex34p, is a peroxisomal integral membrane protein that acts independently and also in concert with the Pex11 protein family members Pex11p, Pex25p, and Pex27p to control the peroxisome populations of cells under conditions of both peroxisome proliferation and constitutive peroxisome division. Yeast two-hybrid analysis showed that Pex34p interacts physically with itself and with Pex11p, Pex25p, and Pex27p but not with Vps1p. Pex34p can act as a positive effector of peroxisome division as its overexpression leads to increased numbers of peroxisomes in wild type and pex34Δ cells. Pex34p requires the Pex11 family proteins to promote peroxisome division. Our discovery of Pex34p as a protein involved in the already complex control of peroxisome populations emphasizes the necessity of cells to strictly regulate their peroxisome populations to be able to respond appropriately to changing environmental conditions.

Links

PubMed PMC3093324 Online version:10.1091/mbc.E11-01-0084

Keywords

Acetyl-CoA C-Acyltransferase/metabolism; Cell Division; Cell Fractionation; Epistasis, Genetic; Gene Deletion; Membrane Proteins/genetics; Membrane Proteins/metabolism; Organelle Shape/genetics; Organelle Size/genetics; Peroxisomes/metabolism; Protein Transport; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/ultrastructure; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:PEX34

located_in

GO:0005779: integral component of peroxisomal membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:PEX34

involved_in

GO:0007031: peroxisome organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PEX34

involved_in

GO:0007031: peroxisome organization

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000005507
SGD:S000005719
SGD:S000006033

P

Seeded From UniProt

complete

YEAST:PEX25

GO:0016559: peroxisome fission

ECO:0000315:

P

Fig 5.

Under conditions of constitutive peroxisome division, deletion of the PEX25 gene led to reductions in the numbers of peroxisomes as compared to wild-type levels.

complete

YEAST:PEX27

GO:0016559: peroxisome fission

ECO:0000315:

P

Fig 5.

Under conditions of constitutive peroxisome division, deletion of the PEX27 gene led to reductions in the numbers of peroxisomes as compared to wild-type levels.

complete


See also

References

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