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PMID:21419340

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Citation

Takahashi, F, Mizoguchi, T, Yoshida, R, Ichimura, K and Shinozaki, K (2011) Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis. Mol. Cell 41:649-60

Abstract

Rapid recognition and signal transduction of mechanical wounding through various signaling molecules, including calcium (Ca²+), protein phosphorylation, and reactive oxygen species (ROS), are necessary early events leading to stress resistance in plants. Here we report that an Arabidopsis mitogen-activated protein kinase 8 (MPK8) connects protein phosphorylation, Ca²+, and ROS in the wound-signaling pathway. MPK8 is activated through mechanical wounding, and this activation requires direct binding of calmodulins (CaMs) in a Ca²+-dependent manner. MPK8 is also phosphorylated and activated by a MAPKK MKK3 in the prototypic kinase cascade, and full activation of MPK8 needs both CaMs and MKK3 in planta. The MPK8 pathway negatively regulates ROS accumulation through controlling expression of the Rboh D gene. These findings suggest that two major activation modes in eukaryotes, Ca²+/CaMs and the MAP kinase phosphorylation cascade, converge at MPK8 to monitor or maintain an essential part of ROS homeostasis.

Links

PubMed Online version:10.1016/j.molcel.2011.02.029

Keywords

Animals; Arabidopsis/anatomy & histology; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Calcium/metabolism; Calmodulin/genetics; Calmodulin/metabolism; Enzyme Activation; Homeostasis/physiology; Hydrogen Peroxide/metabolism; MAP Kinase Kinase 3/genetics; MAP Kinase Kinase 3/metabolism; MAP Kinase Signaling System/physiology; Mitogen-Activated Protein Kinases/metabolism; Oxidants/metabolism; Reactive Oxygen Species/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:M2K3

enables

GO:0004707: MAP kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ZAT12

involved_in

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MPK8

enables

GO:0005516: calmodulin binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT1G66410

F

Seeded From UniProt

complete

ARATH:MPK8

involved_in

GO:0000302: response to reactive oxygen species

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MPK8

involved_in

GO:0009753: response to jasmonic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MPK8

involved_in

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MPK8

involved_in

GO:0042542: response to hydrogen peroxide

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:OXI1

involved_in

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RBOHD

involved_in

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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