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PMID:21395886

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Citation

Lai, Z, Wang, F, Zheng, Z, Fan, B and Chen, Z (2011) A critical role of autophagy in plant resistance to necrotrophic fungal pathogens. Plant J. 66:953-68

Abstract

Autophagy is a pathway for degradation of cytoplasmic components. In plants, autophagy plays an important role in nutrient recycling during nitrogen or carbon starvation, and in responses to abiotic stress. Autophagy also regulates age- and immunity-related programmed cell death, which is important in plant defense against biotrophic pathogens. Here we show that autophagy plays a critical role in plant resistance to necrotrophic pathogens. ATG18a, a critical autophagy protein in Arabidopsis, interacts with WRKY33, a transcription factor that is required for resistance to necrotrophic pathogens. Expression of autophagy genes and formation of autophagosomes are induced in Arabidopsis by the necrotrophic fungal pathogen Botrytis cinerea. Induction of ATG18a and autophagy by B. cinerea was compromised in the wrky33 mutant, which is highly susceptible to necrotrophic pathogens. Arabidopsis mutants defective in autophagy exhibit enhanced susceptibility to the necrotrophic fungal pathogens B. cinerea and Alternaria brassicicola based on increased pathogen growth in the mutants. The hypersusceptibility of the autophagy mutants was associated with reduced expression of the jasmonate-regulated PFD1.2 gene, accelerated development of senescence-like chlorotic symptoms, and increased protein degradation in infected plant tissues. These results strongly suggest that autophagy cooperates with jasmonate- and WRKY33-mediated signaling pathways in the regulation of plant defense responses to necrotrophic pathogens.

Links

PubMed Online version:10.1111/j.1365-313X.2011.04553.x

Keywords

Arabidopsis/genetics; Arabidopsis/immunology; Arabidopsis/metabolism; Arabidopsis/microbiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Autophagy; Binding Sites; Botrytis/immunology; Botrytis/pathogenicity; Cyclopentanes/metabolism; Disease Susceptibility/microbiology; Fluorescence; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Reporter; Immunity, Innate; Oxylipins/metabolism; Plant Diseases/immunology; Plant Diseases/microbiology; Plant Leaves/metabolism; Plants, Genetically Modified/genetics; Plants, Genetically Modified/immunology; Plants, Genetically Modified/metabolism; Plants, Genetically Modified/microbiology; Promoter Regions, Genetic; Recombinant Fusion Proteins/metabolism; Signal Transduction; Transcription Factors/genetics; Transcription Factors/metabolism; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:A8MS84

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATG9

involved_in

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRK33

acts_upstream_of_or_within

GO:0010508: positive regulation of autophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRK33

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q93VB2

F

Seeded From UniProt

complete

ARATH:ATG10

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AT18A

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:AT18A

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:AT18A

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AT18A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8S8P5

F

Seeded From UniProt

complete

ARATH:AT18A

involved_in

GO:0006914: autophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATG7

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATG5

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BECN1

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRK33

involved_in

GO:0010508: positive regulation of autophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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