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PMID:21390509

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Citation

Ohnuma, T, Numata, T, Osawa, T, Mizuhara, M, Lampela, O, Juffer, AH, Skriver, K and Fukamizo, T (2011) A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta 234:123-37

Abstract

Expression of a class V chitinase gene (At4g19810, AtChiC) in Arabidopsis thaliana was examined by quantitative real-time PCR and by analyzing microarray data available at Genevestigator. The gene expression was induced by the plant stress-related hormones abscisic acid (ABA) and jasmonic acid (JA) and by the stress resulting from the elicitor flagellin, NaCl, and osmosis. The recombinant AtChiC protein was produced in E. coli, purified, and characterized with respect to the structure and function. The recombinant AtChiC hydrolyzed N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates. The crystal structure of AtChiC was determined by the molecular replacement method at 2.0 Å resolution. AtChiC was found to adopt an (β/α)(8) fold with a small insertion domain composed of an α-helix and a five-stranded β-sheet. From docking simulation of AtChiC with pentameric substrate, the amino acid residues responsible for substrate binding were found to be well conserved when compared with those of the class V chitinase from Nicotiana tabacum (NtChiV). All of the structural and functional properties of AtChiC are quite similar to those obtained for NtChiV, and seem to be common to class V chitinases from higher plants.

Links

PubMed Online version:10.1007/s00425-011-1390-3

Keywords

Abscisic Acid/adverse effects; Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/metabolism; Chitinase/chemistry; Chitinase/genetics; Chitinase/metabolism; Crystallography, X-Ray; Cyclopentanes/adverse effects; Flagellin/adverse effects; Gene Expression Regulation, Plant; Genes, Plant/physiology; Molecular Sequence Data; Osmosis/physiology; Oxylipins/adverse effects; Plant Growth Regulators/metabolism; Sodium Chloride/adverse effects

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:CHIC

acts_upstream_of_or_within

GO:0006032: chitin catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CHIC

acts_upstream_of_or_within

GO:0009753: response to jasmonic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CHIC

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CHIC

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CHIC

enables

GO:0008843: endochitinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CHIC

enables

GO:0035885: exochitinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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