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PMID:21389053

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Citation

Haralalka, S, Shelton, C, Cartwright, HN, Katzfey, E, Janzen, E and Abmayr, SM (2011) Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. Development 138:1551-62

Abstract

Myoblast fusion is an intricate process that is initiated by cell recognition and adhesion, and culminates in cell membrane breakdown and formation of multinucleate syncytia. In the Drosophila embryo, this process occurs asymmetrically between founder cells that pattern the musculature and fusion-competent myoblasts (FCMs) that account for the bulk of the myoblasts. The present studies clarify and amplify current models of myoblast fusion in several important ways. We demonstrate that the non-conventional guanine nucleotide exchange factor (GEF) Mbc plays a fundamental role in the FCMs, where it functions to activate Rac1, but is not required in the founder cells for fusion. Mbc, active Rac1 and F-actin foci are highly enriched in the FCMs, where they localize to the Sns:Kirre junction. Furthermore, Mbc is crucial for the integrity of the F-actin foci and the FCM cytoskeleton, presumably via its activation of Rac1 in these cells. Finally, the local asymmetric distribution of these proteins at adhesion sites is reminiscent of invasive podosomes and, consistent with this model, they are enriched at sites of membrane deformation, where the FCM protrudes into the founder cell/myotube. These data are consistent with models promoting actin polymerization as the driving force for myoblast fusion.

Links

PubMed PMC3062424 Online version:10.1242/dev.057653

Keywords

Actins/genetics; Actins/metabolism; Animals; Cell Fusion; Cells, Cultured; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Drosophila; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Fluorescent Antibody Technique; Immunohistochemistry; Microscopy, Confocal; Myoblasts/cytology; Myoblasts/metabolism; rac GTP-Binding Proteins/genetics; rac GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:A0A0B4K7Q6

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:A0A0B4K7Q6

located_in

GO:0019897: extrinsic component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:A0A0B4K7Q6

involved_in

GO:0007520: myoblast fusion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:A0A0B4K7Q6

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

F

Seeded From UniProt

complete

DROME:A0A0B4K7Q6

involved_in

GO:0090630: activation of GTPase activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

P

Seeded From UniProt

complete

DROME:RAC1

located_in

GO:0019897: extrinsic component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:RAC1

enables

GO:0003924: GTPase activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0015513

F

Seeded From UniProt

complete

DROME:Q8MQW7

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MQW7

located_in

GO:0019897: extrinsic component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8MQW7

involved_in

GO:0007520: myoblast fusion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MQW7

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

F

Seeded From UniProt

complete

DROME:Q8MQW7

involved_in

GO:0090630: activation of GTPase activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

P

Seeded From UniProt

complete

DROME:Q8MQY7

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MQY7

located_in

GO:0019897: extrinsic component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8MQY7

involved_in

GO:0007520: myoblast fusion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MQY7

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

F

Seeded From UniProt

complete

DROME:Q8MQY7

involved_in

GO:0090630: activation of GTPase activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

P

Seeded From UniProt

complete

DROME:Q9VCH4

involved_in

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VCH4

located_in

GO:0019897: extrinsic component of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VCH4

involved_in

GO:0007520: myoblast fusion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VCH4

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

F

Seeded From UniProt

complete

DROME:Q9VCH4

involved_in

GO:0090630: activation of GTPase activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010333

P

Seeded From UniProt

complete


See also

References

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