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PMID:21371898

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Citation

Tarantino, D, Morandini, P, Ramirez, L, Soave, C and Murgia, I (2011) Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism. Plant Physiol. Biochem. 49:520-9

Abstract

Iron has a major role in mitochondrial as well as in chloroplast metabolism, however the processes involved in organelle iron transport in plants are only partially understood. To identify mitochondrial iron transporters in Arabidopsis, we searched for proteins homologous to the Danio rerio (zebrafish) Mitoferrin2 MFRN2, a mitochondrial iron importer in non-erythroid cells. Among the identified putative Arabidopsis mitoferrinlike proteins, we focused on that one encoded by At5g42130, which we named AtMfl1 (MitoFerrinLike1). AtMfl1 expression strongly correlates with genes coding for proteins involved in chloroplast metabolism. Such an unexpected result is supported by the identification by different research groups, of the protein encoded by At5g42130 and of its homologs from various plant species in the inner chloroplastic envelope membrane proteome. Notably, neither the protein encoded by At5g42130 nor its homologs from other plant species have been identified in the mitochondrial proteome. AtMfl1 gene expression is dependent on Fe supply: AtMfl1 transcript strongly accumulates under Fe excess, moderately under Fe sufficiency and weakly under Fe deficiency. In order to understand the physiological role of AtMfl1, we isolated and characterized two independent AtMfl1 KO mutants, atmfl1-1 and atmfl1-2: both show reduced vegetative growth. When grown under conditions of Fe excess, atmfl1-1 and atmfl1-2 mutants (seedlings, rosette leaves) contain less total Fe than wt and also reduced expression of the iron storage ferritin AtFer1. Taken together, these results suggest that Arabidopsis mitoferrinlike gene AtMfl1 is involved in Fe transport into chloroplasts, under different conditions of Fe supply and that suppression of its expression alters plant Fe accumulation in various developmental stages.

Links

PubMed Online version:10.1016/j.plaphy.2011.02.003

Keywords

Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Transport; Chloroplasts/metabolism; Ferritins/genetics; Ferritins/metabolism; Gene Expression Regulation, Plant; Iron/analysis; Iron/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mitochondria/metabolism; Molecular Sequence Data; Plant Leaves/growth & development; Plant Leaves/metabolism; Seedling/genetics; Seedling/metabolism; Sequence Alignment; Sequence Analysis, Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:MFL1

acts_upstream_of_or_within

GO:0010039: response to iron ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MFL1

acts_upstream_of_or_within

GO:0006826: iron ion transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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