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PMID:21362424

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Citation

Angotzi, AR, Mungpakdee, S, Stefansson, S, Male, R and Chourrout, D (2011) Involvement of Prop1 homeobox gene in the early development of fish pituitary gland. Gen. Comp. Endocrinol. 171:332-40

Abstract

When mutated in mammals, paired-like homeobox Prop1 gene produces highly variable pituitary phenotypes with impaired regulation of Pit1 and eventually defective synthesis of Pit1-regulated pituitary hormones. Here we have identified fish prop1 orthologs, confirmed their pituitary-specific expression, and blocked the splicing of zebrafish prop1 transcripts using morpholino oligonucleotides. Very early steps of the gland formation seemed unaffected based on morphology and expression of early placodal marker pitx. Prop1 knock-down reduced the expression of pit1, prl (prolactin) and gh (growth hormone), as expected if the function of Prop1 is conserved throughout vertebrates. Less expectedly, lim3 was down regulated. This gene is expressed from early stages of vertebrate pituitary development but is not known to be Prop1-dependent. In situ hybridizations on prop1 morphants using probes for the pan pituitary gene pitx3 and for the hormone gene markers prl, gh and tshβ, revealed abnormal shape, growth and cellular organization of the developed adenohypophysis. Strikingly, the effects of prop1 knock-down on adenohypophysis morphology and gene expression were gradually reversed during late development, despite persistent splice-blocking of transcripts. Therefore, prop1 function appears to be conserved between mammals and fish, at least for the mediation of hormonal cell type differentiation via pit1, but the existence of other fish-specific pathways downstream of prop1 are suggested by our observations.

Links

PubMed Online version:10.1016/j.ygcen.2011.02.026

Keywords

Animals; Homeodomain Proteins/chemistry; Homeodomain Proteins/classification; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; In Situ Hybridization; Phylogeny; Pituitary Gland/metabolism; Polymerase Chain Reaction; Salmon; Thyrotropin, beta Subunit/metabolism; Transcription Factor Pit-1/chemistry; Transcription Factor Pit-1/classification; Transcription Factor Pit-1/genetics; Transcription Factor Pit-1/metabolism; Zebrafish; Zebrafish Proteins/chemistry; Zebrafish Proteins/classification; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A2R8QKN2

involved_in

GO:0021984: adenohypophysis development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110620-1

P

Seeded From UniProt

complete

DANRE:A0A2R8QNY0

involved_in

GO:0021984: adenohypophysis development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110620-1

P

Seeded From UniProt

complete

DANRE:B8R403

involved_in

GO:0021984: adenohypophysis development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110620-1

P

Seeded From UniProt

complete

DANRE:F6PE50

involved_in

GO:0021984: adenohypophysis development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110620-1

P

Seeded From UniProt

complete


See also

References

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