GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:21354322

From GONUTS
Jump to: navigation, search
Citation

Aldeco, M, Arslan, BK and Edmondson, DE (2011) Catalytic and inhibitor binding properties of zebrafish monoamine oxidase (zMAO): comparisons with human MAO A and MAO B. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 159:78-83

Abstract

A comparative investigation of substrate specificity and inhibitor binding properties of recombinant zebrafish (Danio rerio) monoamine oxidase (zMAO) with those of recombinant human monoamine oxidases A and B (hMAO A and hMAO B) is presented. zMAO oxidizes the neurotransmitter amines (serotonin, dopamine and tyramine) with k(cat) values that exceed those of hMAO A or of hMAO B. The enzyme is competitively inhibited by hMAO A selective reversible inhibitors with the exception of d-amphetamine where uncompetitive inhibition is exhibited. The enzyme is unreactive with most MAO B-specific reversible inhibitors with the exception of chlorostyrylcaffeine. zMAO catalyzes the oxidation of para-substituted benzylamine analogs exhibiting (D)k(cat) and (D)(k(cat)/K(m)) values ranging from 2 to 8. Structure-activity correlations show a dependence of log k(cat) with the electronic factor σ(p) with a ρ value of +1.55±0.34; a value close to that for hMAO A but not with MAO B. zMAO differs from hMAO A or hMAO B in benzylamine analog binding correlations where an electronic effect (ρ=+1.29±0.31) is observed. These data demonstrate zMAO exhibits functional properties similar to hMAO A as well as exhibits its own unique behavior. These results should be useful for studies of MAO function in zebrafish models of human disease states.

Links

PubMed PMC3071876 Online version:10.1016/j.cbpb.2011.02.002

Keywords

Animals; Benzylamines/metabolism; Binding Sites; Biocatalysis/drug effects; Dextroamphetamine/chemistry; Dextroamphetamine/pharmacology; Dopamine/metabolism; Gene Expression; Humans; Isoenzymes/genetics; Isoenzymes/metabolism; Kinetics; Monoamine Oxidase/genetics; Monoamine Oxidase/metabolism; Monoamine Oxidase Inhibitors/chemistry; Monoamine Oxidase Inhibitors/pharmacology; Oxidation-Reduction; Pichia; Protein Binding; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Serotonin/metabolism; Species Specificity; Substrate Specificity; Tyramine/metabolism; Zebrafish/genetics; Zebrafish/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:AOF

enables

GO:0008131: primary amine oxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:AOF

involved_in

GO:0042402: cellular biogenic amine catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.