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PMID:21350010

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Citation

Van de Bor, V, Zimniak, G, Cérézo, D, Schaub, S and Noselli, S (2011) Asymmetric localisation of cytokine mRNA is essential for JAK/STAT activation during cell invasiveness. Development 138:1383-93

Abstract

The transition from immotile epithelial cells to migrating cells occurs in all organisms during normal embryonic development, as well as during tumour metastasis. During Drosophila oogenesis, border cells (BCs) are recruited and delaminate from the follicular epithelium. This process is triggered by the polar cells (PCs), which secrete the cytokine Unpaired (Upd) and activate the JAK/STAT pathway in neighbouring cells, turning them into invasive BCs. Interestingly, either a decrease or an increase in BC number alters migration, indicating that mechanisms controlling the level of JAK/STAT signalling are crucial in this process. Here, we show that PCs have a highly stable and polarised network of microtubules along which upd transcripts are asymmetrically transported in a Dynein-dependent manner. We demonstrate that in the absence of upd mRNA localisation the ligand is no longer efficiently secreted, leading to a loss of signalling strength as well as recruitment and migration defects. These findings reveal a novel post-transcriptional regulatory mechanism of JAK/STAT signalling in the control of epithelial cell invasiveness.

Links

PubMed Online version:10.1242/dev.056184

Keywords

Animals; Cell Movement/physiology; Cytokines/genetics; Cytokines/metabolism; Drosophila; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Female; Immunohistochemistry; In Situ Hybridization; Janus Kinases/genetics; Janus Kinases/metabolism; Microtubules/genetics; Microtubules/metabolism; Oogenesis/physiology; RNA, Messenger/genetics; RNA, Messenger/metabolism; STAT Transcription Factors/genetics; STAT Transcription Factors/metabolism; Signal Transduction/physiology; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O96525

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O96525

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8MZC7

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8MZC7

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VWW4

located_in

GO:0045177: apical part of cell

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VWW4

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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