GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:21345178
Citation |
Gao, H, Zhang, Y, Han, Y, Yang, L, Liu, X, Guo, Z, Tan, Y, Huang, X, Zhou, D and Yang, R (2011) Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis. BMC Microbiol. 11:39 |
---|---|
Abstract |
The osmotic regulator OmpR in Escherichia coli regulates differentially the expression of major porin proteins OmpF and OmpC. In Yersinia enterocolitica and Y. pseudotuberculosis, OmpR is required for both virulence and survival within macrophages. However, the phenotypic and regulatory roles of OmpR in Y. pestis are not yet fully understood. |
Links |
PubMed PMC3050692 Online version:10.1186/1471-2180-11-39 |
Keywords |
Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Line; DNA, Bacterial/genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Macrophages/microbiology; Mice; Mutation; Oligonucleotide Array Sequence Analysis; Phagocytosis; Phenotype; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic; Yersinia pestis/genetics; Yersinia pestis/metabolism |
edit table |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0090046: regulation of transcription regulator activity |
IMP: Inferred from Mutant Phenotype: |
P |
OmpR was found to have an effect on 224 genes based on cDNA microarray data, representing 4% of the proteins in the cell. 16 genes were validated to have OmpR-dependent transcription, including ompR, ompC, ompF, and ompX, which were further classified. Figure 2 gives a comparison of mRNA levels between WT and ompR mutant using RT-PCR and LacZ fusion. OmpC, ompF, and ompZ show between a 3 and 25-fold decrease in expression in the mutant. |
complete |
| |||
GO:0006950: response to stress |
IMP: Inferred from Mutant Phenotype: |
P |
Figure 1 shows a decreased survival when exposed to high osmolarity, high temperature, and low pH for the ompR mutant as well as a higher rate of phagocytosis. |
complete |
| |||
See also
References
See Help:References for how to manage references in GONUTS.