GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:21345178

From GONUTS
Jump to: navigation, search
Citation

Gao, H, Zhang, Y, Han, Y, Yang, L, Liu, X, Guo, Z, Tan, Y, Huang, X, Zhou, D and Yang, R (2011) Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis. BMC Microbiol. 11:39

Abstract

The osmotic regulator OmpR in Escherichia coli regulates differentially the expression of major porin proteins OmpF and OmpC. In Yersinia enterocolitica and Y. pseudotuberculosis, OmpR is required for both virulence and survival within macrophages. However, the phenotypic and regulatory roles of OmpR in Y. pestis are not yet fully understood.

Links

PubMed PMC3050692 Online version:10.1186/1471-2180-11-39

Keywords

Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Line; DNA, Bacterial/genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Macrophages/microbiology; Mice; Mutation; Oligonucleotide Array Sequence Analysis; Phagocytosis; Phenotype; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription, Genetic; Yersinia pestis/genetics; Yersinia pestis/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YERPG:A9R4C4

GO:0090046: regulation of transcription regulator activity

IMP: Inferred from Mutant Phenotype:

P

OmpR was found to have an effect on 224 genes based on cDNA microarray data, representing 4% of the proteins in the cell. 16 genes were validated to have OmpR-dependent transcription, including ompR, ompC, ompF, and ompX, which were further classified. Figure 2 gives a comparison of mRNA levels between WT and ompR mutant using RT-PCR and LacZ fusion. OmpC, ompF, and ompZ show between a 3 and 25-fold decrease in expression in the mutant.

complete


YERPG:A9R4C4

GO:0006950: response to stress

IMP: Inferred from Mutant Phenotype:

P

Figure 1 shows a decreased survival when exposed to high osmolarity, high temperature, and low pH for the ompR mutant as well as a higher rate of phagocytosis.

complete



See also

References

See Help:References for how to manage references in GONUTS.