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PMID:21307598

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Citation

Kim, DW, Kim, KB, Kim, JY and Seo, SB (2011) Characterization of a novel histone H3K36 methyltransferase setd3 in zebrafish. Biosci. Biotechnol. Biochem. 75:289-94

Abstract

Post-translational modifications of histones have been demonstrated to play important roles in the regulation of chromatin structure and transcriptional regulation. In histone modification, methylated lysine has an important role in transcriptional regulation. The evolutionarily conserved SET domain was first identified in Drosophila proteins: Suppressor of variegation (Su(var)3-9), Enhancer of zeste (E(z)), and Trithorax. SET domain-containing proteins have histone methyltransferase (HMTase) activity via the SET domain. Using a bioinformatics approach, we identified and cloned zebrafish setd3 containing SET and Rubis-subs-bind domains. In this study, we report that setd3 had lysine specificity toward histone H3K36. Methylation of histone H3K36 is known as one of the transcriptional activation markers. It transiently transfected setd3 activated general transcription in reporter assays. Overexpression of setd3 decreased cell viability and activated caspase-3, indicating possible roles in apoptotic cell death and cell cycle regulation.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Caspase 3/metabolism; Cell Survival; Conserved Sequence; Enzyme Activation; Histone-Lysine N-Methyltransferase/chemistry; Histone-Lysine N-Methyltransferase/genetics; Histone-Lysine N-Methyltransferase/metabolism; Lysine/metabolism; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Transcriptional Activation; Zebrafish; Zebrafish Proteins/chemistry; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:SETD3

involved_in

GO:0018027: peptidyl-lysine dimethylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

involved_in

GO:0018026: peptidyl-lysine monomethylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

involved_in

GO:0018023: peptidyl-lysine trimethylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

involved_in

GO:0001558: regulation of cell growth

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

involved_in

GO:0010452: histone H3-K36 methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

involved_in

GO:0008283: cell population proliferation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

enables

GO:0003713: transcription coactivator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:SETD3

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:SETD3

enables

GO:0046975: histone methyltransferase activity (H3-K36 specific)

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:SETD3

enables

GO:0046975: histone methyltransferase activity (H3-K36 specific)

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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