GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:21306299

From GONUTS
Jump to: navigation, search
Citation

Heikkilä, E, Ristola, M, Havana, M, Jones, N, Holthöfer, H and Lehtonen, S (2011) Trans-interaction of nephrin and Neph1/Neph3 induces cell adhesion that associates with decreased tyrosine phosphorylation of nephrin. Biochem. J. 435:619-28

Abstract

Slit diaphragms are specialized junctions between glomerular epithelial cells (podocytes) that are crucial for glomerular ultrafiltration. The Ig superfamily members nephrin and Neph1 are essential components of the slit diaphragm, whereas the role of Neph1 homologue Neph3 in the slit diaphragm is unknown. In the present paper we show that Neph3 homodimerizes and heterodimerizes with nephrin and Neph1. We further investigated whether these interactions play a role in cell adhesion by using mouse L fibroblasts that lack endogenous cell-adhesion activity and found that Neph1 and Neph3 are able to induce cell adhesion alone, whereas nephrin needs to trans-interact with Neph1 or Neph3 in order to promote formation of cell-cell contacts. Tyrosine phosphorylation of nephrin was down-regulated after nephrin trans-interacted with either Neph1 or Neph3 leading to formation of cell-cell contacts. We further found that the expression of Neph3 was increased in nephrin-deficient mouse podocytes. The findings of the present paper show that nephrin and Neph1 or Neph3 trans-interactions promote cell-contact formation, suggesting that they may also function together in slit diaphragm assembly.

Links

PubMed Online version:10.1042/BJ20101599

Keywords

Animals; Cell Adhesion; Cell Line; Gene Expression Regulation/physiology; Humans; Immunoglobulins/genetics; Immunoglobulins/metabolism; Kidney/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Rats; Tyrosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:KIRR2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9QY53

F

Seeded From UniProt

complete

MOUSE:KIRR2

acts_upstream_of_or_within

GO:0001933: negative regulation of protein phosphorylation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1891396

P

Seeded From UniProt

complete

MOUSE:KIRR2

located_in

GO:0036057: slit diaphragm

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000653)

Seeded From UniProt

complete

MOUSE:KIRR2

located_in

GO:0005911: cell-cell junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:KIRR2

acts_upstream_of_or_within

GO:0098609: cell-cell adhesion

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1859637
MGI:MGI:1891396

P

Seeded From UniProt

complete

MOUSE:KIRR1

acts_upstream_of_or_within

GO:0001933: negative regulation of protein phosphorylation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:2442334

P

Seeded From UniProt

complete

MOUSE:KIRR1

located_in

GO:0005911: cell-cell junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:KIRR1

acts_upstream_of_or_within

GO:0098609: cell-cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:KIRR1

acts_upstream_of_or_within

GO:0098609: cell-cell adhesion

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1859637
MGI:MGI:2442334

P

Seeded From UniProt

complete

MOUSE:NPHN

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q7TSU7

F

Seeded From UniProt

complete

MOUSE:NPHN

acts_upstream_of_or_within

GO:0007155: cell adhesion

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1891396
MGI:MGI:2442334

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.