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PMID:21299656

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Citation

Renninger, SL, Gesemann, M and Neuhauss, SC (2011) Cone arrestin confers cone vision of high temporal resolution in zebrafish larvae. Eur. J. Neurosci. 33:658-67

Abstract

Vision of high temporal resolution depends on careful regulation of photoresponse kinetics, beginning with the lifetime of activated photopigment. The activity of rhodopsin is quenched by high-affinity binding of arrestin to photoexcited phosphorylated photopigment, which effectively terminates the visual transduction cascade. This regulation mechanism is well established for rod photoreceptors, yet its role for cone vision is still controversial. In this study we therefore analyzed arrestin function in the cone-dominated vision of larval zebrafish. For both rod (arrS ) and cone (arr3 ) arrestin we isolated two paralogs, each expressed in the respective subset of photoreceptors. Labeling with paralog-specific antibodies revealed subfunctionalized expression of Arr3a in M- and L-cones, and Arr3b in S- and UV-cones. The inactivation of arr3a by morpholino knockdown technology resulted in a severe delay in photoresponse recovery which, under bright light conditions, was rate-limiting. Comparison to opsin phosphorylation-deficient animals confirmed the role of cone arrestin in late cone response recovery. Arr3a activity partially overlapped with the function of the cone-specific kinase Grk7a involved in initial response recovery. Behavioral measurements further revealed Arr3a deficiency to be sufficient to reduce temporal contrast sensitivity, providing evidence for the importance of arrestin in cone vision of high temporal resolution.

Links

PubMed Online version:10.1111/j.1460-9568.2010.07574.x

Keywords

Animals; Arrestin/classification; Arrestin/genetics; Arrestin/metabolism; Electroretinography; Gene Knockdown Techniques; Larva/anatomy & histology; Larva/physiology; Protein Isoforms/genetics; Protein Isoforms/metabolism; Retinal Cone Photoreceptor Cells/cytology; Retinal Cone Photoreceptor Cells/physiology; Vision, Ocular/physiology; Zebrafish/anatomy & histology; Zebrafish/embryology; Zebrafish/metabolism; Zebrafish Proteins/classification; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A2R8RI04

involved_in

GO:0007601: visual perception

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110303-1

P

Seeded From UniProt

complete

DANRE:Q49HM7

involved_in

GO:0007601: visual perception

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110303-1

P

Seeded From UniProt

complete

DANRE:GRK7A

involved_in

GO:0007601: visual perception

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050824-1

P

Seeded From UniProt

complete

DANRE:Q6DH07

involved_in

GO:0007601: visual perception

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110303-1

P

Seeded From UniProt

complete


See also

References

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