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PMID:21298005

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Citation

Chatterjee, N, Rollins, J, Mahowald, AP and Bazinet, C (2011) Neurotransmitter Transporter-Like: a male germline-specific SLC6 transporter required for Drosophila spermiogenesis. PLoS ONE 6:e16275

Abstract

The SLC6 class of membrane transporters, known primarily as neurotransmitter transporters, is increasingly appreciated for its roles in nutritional uptake of amino acids and other developmentally specific functions. A Drosophila SLC6 gene, Neurotransmitter transporter-like (Ntl), is expressed only in the male germline. Mobilization of a transposon inserted near the 3' end of the Ntl coding region yields male-sterile mutants defining a single complementation group. Germline transformation with Ntl cDNAs under control of male germline-specific control elements restores Ntl/Ntl homozygotes to normal fertility, indicating that Ntl is required only in the germ cells. In mutant males, sperm morphogenesis appears normal, with elongated, individualized and coiled spermiogenic cysts accumulating at the base of the testes. However, no sperm are transferred to the seminal vesicle. The level of polyglycylation of Ntl mutant sperm tubulin appears to be significantly lower than that of wild type controls. Glycine transporters are the most closely related SLC6 transporters to Ntl, suggesting that Ntl functions as a glycine transporter in developing sperm, where augmentation of the cytosolic pool of glycine may be required for the polyglycylation of the massive amounts of tubulin in the fly's giant sperm. The male-sterile phenotype of Ntl mutants may provide a powerful genetic system for studying the function of an SLC6 transporter family in a model organism.

Links

PubMed PMC3029318 Online version:10.1371/journal.pone.0016275

Keywords

Animals; Drosophila Proteins/physiology; Drosophila melanogaster/cytology; Drosophila melanogaster/physiology; Fertility; Glycine Plasma Membrane Transport Proteins/physiology; Male; Neurotransmitter Transport Proteins/physiology; Phenotype; Spermatogenesis; Spermatozoa/chemistry; Spermatozoa/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q8IPH3

involved_in

GO:0030317: flagellated sperm motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IPH3

enables

GO:0015187: glycine transmembrane transporter activity

ECO:0000250: sequence similarity evidence used in manual assertion

HGNC:11051

F

Seeded From UniProt

complete

DROME:Q8IPH3

enables

GO:0015187: glycine transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:Q8IPH3

involved_in

GO:1903804: glycine import across plasma membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8IPH3

involved_in

GO:1903346: positive regulation of protein polyglycylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MZA1

involved_in

GO:0030317: flagellated sperm motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MZA1

enables

GO:0015187: glycine transmembrane transporter activity

ECO:0000250: sequence similarity evidence used in manual assertion

HGNC:11051

F

Seeded From UniProt

complete

DROME:Q8MZA1

enables

GO:0015187: glycine transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:Q8MZA1

involved_in

GO:1903804: glycine import across plasma membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8MZA1

involved_in

GO:1903346: positive regulation of protein polyglycylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VLY6

involved_in

GO:0030317: flagellated sperm motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VLY6

enables

GO:0015187: glycine transmembrane transporter activity

ECO:0000250: sequence similarity evidence used in manual assertion

HGNC:11051

F

Seeded From UniProt

complete

DROME:Q9VLY6

enables

GO:0015187: glycine transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:Q9VLY6

involved_in

GO:1903804: glycine import across plasma membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VLY6

involved_in

GO:1903346: positive regulation of protein polyglycylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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