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PMID:21293028

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Citation

Yang, Y and Lu, X (2011) Drosophila sperm motility in the reproductive tract. Biol. Reprod. 84:1005-15

Abstract

Motile cilia and flagella exhibit many waveforms as outputs of dynein activation sequences on the highly conserved axoneme. Motility change of sperm in the reproductive tract is difficult to study and remains an important area of investigation. Sperm typically execute a sinusoidal waveform. Increased viscosity in the medium induces somewhat unusual arc-line and helical waveforms in some sperm. However, whether the latter two waveforms occur in vivo is not known. Using green fluorescence protein imaging, we show that Drosophila sperm in the uterus move in circular foci via arc-line waves, predominantly in a tail-leading orientation. From the uterus, a small fraction of the sperm enters the seminal receptacle (SR) in parallel formations. After sperm storage and coincident with fertilization of the egg, the sperm exit the SR via head-leading helical waves. Consistent with the observed bidirectional movements, the sperm show the ability to propagate both base-to-tip and tip-to-base flagellar waves. Numerous studies have shown that sperm motility is regulated by intraflagellar calcium concentrations; in particular, the Pkd2 calcium channel has been shown to affect sperm storage. Our analyses here suggest that Pkd2 is required for the sperm to adopt the correct waveform and movement orientation during SR entry. A working model for the sperm's SR entry movement is proposed.

Links

PubMed PMC3080424 Online version:10.1095/biolreprod.110.088773

Keywords

Adnexa Uteri/physiology; Animals; Animals, Genetically Modified; Drosophila melanogaster/physiology; Female; Fertilization; Genes, Reporter; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Image Processing, Computer-Assisted; Imaging, Three-Dimensional; Insect Proteins/metabolism; Male; Microscopy, Video; Models, Biological; Protein Kinases/metabolism; Semen Analysis/methods; Sperm Motility; Sperm Tail/physiology; Sperm Transport; Spermatozoa/enzymology; Spermatozoa/physiology; Uterus/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q6T3S5

involved_in

GO:0030317: flagellated sperm motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VK95

involved_in

GO:0030317: flagellated sperm motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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