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PMID:21288957

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Citation

Pakes, NK, Jayasinghe, SN and Williams, RS (2011) Bio-electrospraying and aerodynamically assisted bio-jetting the model eukaryotic Dictyostelium discoideum: assessing stress and developmental competency post treatment. J R Soc Interface 8:1185-91

Abstract

Bio-electrospraying (BES) and aerodynamically assisted bio-jetting (AABJ) have recently been established as important novel biospray technologies for directly manipulating living cells. To elucidate their potential in medical and clinical sciences, these bio-aerosol techniques have been subjected to increasingly rigorous investigations. In parallel to these studies, we wish to introduce these unique biotechnologies for use in the basic biological sciences, for handling a wide range of cell types and systems, thus increasing the range and the scope of these techniques for modern research. Here, the authors present the analysis of the new use of these biospray techniques for the direct handling of the simple eukaryotic biomedical model organism Dictyostelium discoideum. These cells are widely used as a model for immune cell chemotaxis and as a simple model for development. We demonstrate that AABJ of these cells did not cause cell stress, as defined by the stress-gene induction, nor affect cell development. Furthermore, although BES induced the increased expression of one stress-related gene (gapA), this was not a generalized stress response nor did it affect cell development. These data suggest that these biospray techniques can be used to directly manipulate single cells of this biomedical model without inducing a generalized stress response or perturbing later development.

Links

PubMed PMC3119884 Online version:10.1098/rsif.2010.0696

Keywords

Aerosols; Dictyostelium/physiology; Gene Expression Regulation/physiology; Humans; Models, Immunological; Protozoan Proteins/biosynthesis; Stress, Physiological/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:YAKA

acts_upstream_of_or_within

GO:0006972: hyperosmotic response

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:RTOA

acts_upstream_of_or_within

GO:0009408: response to heat

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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