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PMID:21285328

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Citation

Borg, M, Brownfield, L, Khatab, H, Sidorova, A, Lingaya, M and Twell, D (2011) The R2R3 MYB transcription factor DUO1 activates a male germline-specific regulon essential for sperm cell differentiation in Arabidopsis. Plant Cell 23:534-49

Abstract

The male germline in flowering plants arises through asymmetric division of a haploid microspore. The resulting germ cell undergoes mitotic division and specialization to produce the two sperm cells required for double fertilization. The male germline-specific R2R3 MYB transcription factor DUO1 POLLEN1 (DUO1) plays an essential role in sperm cell specification by activating a germline-specific differentiation program. Here, we show that ectopic expression of DUO1 upregulates a significant number (~63) of germline-specific or enriched genes, including those required for fertilization. We validated 14 previously unknown DUO1 target genes by demonstrating DUO1-dependent promoter activity in the male germline. DUO1 is shown to directly regulate its target promoters through binding to canonical MYB sites, suggesting that the DUO1 target genes validated thus far are likely to be direct targets. This work advances knowledge of the DUO1 regulon that encompasses genes with a range of cellular functions, including transcription, protein fate, signaling, and transport. Thus, the DUO1 regulon has a major role in shaping the germline transcriptome and functions to commit progenitor germ cells to sperm cell differentiation.

Links

PubMed PMC3077786 Online version:10.1105/tpc.110.081059

Keywords

Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Computational Biology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genetic Complementation Test; Oligonucleotide Array Sequence Analysis; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Pollen/growth & development; Promoter Regions, Genetic; Regulon; Reproduction; Tobacco/genetics; Tobacco/growth & development; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:DUO1

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:DUO1

involved_in

GO:0048235: pollen sperm cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DUO1

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DUO1

enables

GO:0043565: sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:F4I5A3

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:F4JN30

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HAP2

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GEX2

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ZAT3

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:O81801

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:IMPA8

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:OPT8

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DMP9

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:H3L2

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Q9LZ87

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:FB215

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ZAT2

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:M3K20

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Q9SR12

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TIP51

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PCR11

acts_upstream_of_or_within

GO:0048235: pollen sperm cell differentiation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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