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PMID:21278126

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Citation

Kim, HJ, Ok, SH, Bahn, SC, Jang, J, Oh, SA, Park, SK, Twell, D, Ryu, SB and Shin, JS (2011) Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination. Plant Cell 23:94-110

Abstract

The phospholipase A(2) (PLA(2)) superfamily of lipolytic enzymes is involved in a number of essential biological processes, such as inflammation, development, host defense, and signal transduction. Despite the proven involvement of plant PLA(2)s in many biological functions, including senescence, wounding, elicitor and stress responses, and pathogen defense, relatively little is known about plant PLA(2)s, and their genes essentially remain uncharacterized. We characterized three of four Arabidopsis thaliana PLA(2) paralogs (PLA(2)-β, -γ, and -δ) and found that they (1) are expressed during pollen development, (2) localize to the endoplasmic reticulum and/or Golgi, and (3) play critical roles in pollen development and germination and tube growth. The suppression of PLA(2) using the RNA interference approach resulted in pollen lethality. The inhibition of pollen germination by pharmacological PLA(2) inhibitors was rescued by a lipid signal molecule, lysophosphatidyl ethanolamine. Based on these results, we propose that plant reproduction, in particular, male gametophyte development, requires the activities of the lipid-modifying PLA(2)s that are conserved in other organisms.

Links

PubMed PMC3051258 Online version:10.1105/tpc.110.074799

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Endoplasmic Reticulum/enzymology; Gene Expression Profiling; Gene Expression Regulation, Plant; Germination; Golgi Apparatus/enzymology; Lysophospholipids/metabolism; Mutation; Phospholipases A2/genetics; Phospholipases A2/metabolism; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/genetics; Pollen/genetics; Pollen/growth & development; Pollen/ultrastructure; RNA Interference; RNA, Plant/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4ITF6

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2D

involved_in

GO:0009846: pollen germination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2D

involved_in

GO:0009860: pollen tube growth

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2D

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PLA2D

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2B

involved_in

GO:0009846: pollen germination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2B

involved_in

GO:0009860: pollen tube growth

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2C

involved_in

GO:0009846: pollen germination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2C

involved_in

GO:0009860: pollen tube growth

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PLA2C

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PLA2C

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PLA2C

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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