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PMID:21275995

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Citation

Zhang, Y, Xiao, Y, Du, F, Cao, L, Dong, H and Ren, H (2011) Arabidopsis VILLIN4 is involved in root hair growth through regulating actin organization in a Ca2+-dependent manner. New Phytol. 190:667-82

Abstract

• Villin is one of the major actin filament bundling proteins in plants. The function of Arabidopsis VILLINs (AtVLNs) is still poorly understood in living cells. In this report, the biochemical activity and cellular function of AtVLN4 were examined. • The biochemical property of AtVLN4 was characterized by co-sedimentation assays, fluorescence microscopy and spectroscopy of pyrene fluorescence. The in vivo function of AtVLN4 was analysed by ectopically expressing it in tobacco pollen and examining the phenotypes of its T-DNA insertional plants. • Recombinant AtVLN4 protein exhibited multiple activities on actin, including actin filament bundling, calcium (Ca(2+))-dependent filament severing and barbed end capping. Expression of AtVLN4 in tobacco pollen induced the formation of supernumerary actin cables and reduced pollen tube growth. Loss of function of AtVLN4 resulted in slowing of root hair growth, alteration in cytoplasmic streaming routes and rate, and reduction of both axial and apical actin bundles. • Our results demonstrated that AtVLN4 is involved in root hair growth through regulating actin organization in a Ca(2+)-dependent manner.

Links

PubMed Online version:10.1111/j.1469-8137.2010.03632.x

Keywords

Actin Cytoskeleton/metabolism; Actins/metabolism; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Calcium/metabolism; Cytoplasmic Streaming; DNA, Bacterial/genetics; Genes, Plant/genetics; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Mutagenesis, Insertional/genetics; Mutation/genetics; Phenotype; Plant Roots/growth & development; Plant Roots/metabolism; Pollen Tube/growth & development; Pollen Tube/metabolism; Protein Binding; Protein Transport; Tobacco/growth & development; Tobacco/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:VILI4

acts_upstream_of_or_within

GO:0030042: actin filament depolymerization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VILI4

colocalizes_with

GO:0005884: actin filament

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:VILI4

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VILI4

acts_upstream_of_or_within

GO:0051014: actin filament severing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VILI4

acts_upstream_of_or_within

GO:0048767: root hair elongation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VILI4

acts_upstream_of_or_within

GO:0099636: cytoplasmic streaming

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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