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Zhang, Z, Chen, H, Huang, X, Xia, R, Zhao, Q, Lai, J, Teng, K, Li, Y, Liang, L, Du, Q, Zhou, X, Guo, H and Xie, Q (2011) BSCTV C2 attenuates the degradation of SAMDC1 to suppress DNA methylation-mediated gene silencing in Arabidopsis. Plant Cell 23:273-88


Plant viruses are excellent tools for studying microbial-plant interactions as well as the complexities of host activities. Our study focuses on the role of C2 encoded by Beet severe curly top virus (BSCTV) in the virus-plant interaction. Using BSCTV C2 as bait in a yeast two-hybrid screen, a C2-interacting protein, S-adenosyl-methionine decarboxylase 1 (SAMDC1), was identified from an Arabidopsis thaliana cDNA library. The interaction was confirmed by an in vitro pull-down assay and a firefly luciferase complemention imaging assay in planta. Biochemical analysis further showed that the degradation of the SAMDC1 protein was inhibited by MG132, a 26S proteasome inhibitor, and that C2 could attenuate the degradation of the SAMDC1 protein. Genetic analysis showed that loss of function of SAMDC1 resulted in reduced susceptibility to BSCTV infection and reduced viral DNA accumulation, similar to the effect of BSCTV C2 deficiency. Bisulfite sequencing analysis further showed that C2 deficiency caused enhanced DNA methylation of the viral genome in infected plants. We also showed that C2 can suppress de novo methylation in the FWA transgenic assay in the C2 transgene background. Overexpression of SAMDC1 can mimic the suppressive activity of C2 against green fluorescent protein-directed silencing. These results suggest that C2 interferes with the host defense mechanism of DNA methylation-mediated gene silencing by attenuating the 26S proteasome-mediated degradation of SAMDC1.


PubMed PMC3051253 Online version:10.1105/tpc.110.081695


Adenosylmethionine Decarboxylase/chemistry; Arabidopsis/enzymology; Arabidopsis/virology; DNA Methylation; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Geminiviridae/genetics; Geminiviridae/metabolism; Gene Silencing; Genome, Viral; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/virology; Two-Hybrid System Techniques; Viral Proteins/genetics; Viral Proteins/metabolism



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion



Seeded From UniProt




GO:0019079: viral genome replication

ECO:0000315: mutant phenotype evidence used in manual assertion


Seeded From UniProt


See also


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