GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:21245466

From GONUTS
Jump to: navigation, search
Citation

Zhang, Z, Chen, H, Huang, X, Xia, R, Zhao, Q, Lai, J, Teng, K, Li, Y, Liang, L, Du, Q, Zhou, X, Guo, H and Xie, Q (2011) BSCTV C2 attenuates the degradation of SAMDC1 to suppress DNA methylation-mediated gene silencing in Arabidopsis. Plant Cell 23:273-88

Abstract

Plant viruses are excellent tools for studying microbial-plant interactions as well as the complexities of host activities. Our study focuses on the role of C2 encoded by Beet severe curly top virus (BSCTV) in the virus-plant interaction. Using BSCTV C2 as bait in a yeast two-hybrid screen, a C2-interacting protein, S-adenosyl-methionine decarboxylase 1 (SAMDC1), was identified from an Arabidopsis thaliana cDNA library. The interaction was confirmed by an in vitro pull-down assay and a firefly luciferase complemention imaging assay in planta. Biochemical analysis further showed that the degradation of the SAMDC1 protein was inhibited by MG132, a 26S proteasome inhibitor, and that C2 could attenuate the degradation of the SAMDC1 protein. Genetic analysis showed that loss of function of SAMDC1 resulted in reduced susceptibility to BSCTV infection and reduced viral DNA accumulation, similar to the effect of BSCTV C2 deficiency. Bisulfite sequencing analysis further showed that C2 deficiency caused enhanced DNA methylation of the viral genome in infected plants. We also showed that C2 can suppress de novo methylation in the FWA transgenic assay in the C2 transgene background. Overexpression of SAMDC1 can mimic the suppressive activity of C2 against green fluorescent protein-directed silencing. These results suggest that C2 interferes with the host defense mechanism of DNA methylation-mediated gene silencing by attenuating the 26S proteasome-mediated degradation of SAMDC1.

Links

PubMed PMC3051253 Online version:10.1105/tpc.110.081695

Keywords

Adenosylmethionine Decarboxylase/chemistry; Arabidopsis/enzymology; Arabidopsis/virology; DNA Methylation; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Geminiviridae/genetics; Geminiviridae/metabolism; Gene Silencing; Genome, Viral; Plants, Genetically Modified/enzymology; Plants, Genetically Modified/virology; Two-Hybrid System Techniques; Viral Proteins/genetics; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:DCAM1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

NCBI_gi:2982475

F

Seeded From UniProt

complete

ARATH:DCAM1

acts_upstream_of_or_within

GO:0019079: viral genome replication

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.