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PMID:2121614

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Citation

Lowe, N, Brady, HJ, Barlow, JH, Sowden, JC, Edwards, M and Butterworth, PH (1990) Structure and methylation patterns of the gene encoding human carbonic anhydrase I. Gene 93:277-83

Abstract

The gene (CAI) encoding human carbonic anhydrase I (CAI) has been isolated and shown to have a total length of 50 kb. Some 36 kb of this consists of a large intron separating the erythroid-specific promoter from the coding region. A small (54 bp) noncoding exon from within this intron is occasionally found in transcripts. Two different polyadenylation sites have been found, the most distal of which is the most commonly used. Methylation levels near the promoter differ widely in cell lines. In CAI-expressing cells, a region of DNA near the promoter is demethylated in a generally highly methylated background. Surprisingly, non-CAI-expressing cell lines show much lower levels of methylation.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; Carbonic Anhydrases/biosynthesis; Carbonic Anhydrases/genetics; DNA/chemistry; DNA-Binding Proteins/genetics; Erythroid-Specific DNA-Binding Factors; Exons; HeLa Cells; Humans; Methylation; Molecular Sequence Data; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Restriction Mapping; TATA Box; Transcription Factors/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:CAH1

enables

GO:0004089: carbonate dehydratase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

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