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PMID:21205795

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Citation

Aguirre-Chen, C, Bülow, HE and Kaprielian, Z (2011) C. elegans bicd-1, homolog of the Drosophila dynein accessory factor Bicaudal D, regulates the branching of PVD sensory neuron dendrites. Development 138:507-18

Abstract

The establishment of cell type-specific dendritic arborization patterns is a key phase in the assembly of neuronal circuitry that facilitates the integration and processing of synaptic and sensory input. Although studies in Drosophila and vertebrate systems have identified a variety of factors that regulate dendrite branch formation, the molecular mechanisms that control this process remain poorly defined. Here, we introduce the use of the Caenorhabditis elegans PVD neurons, a pair of putative nociceptors that elaborate complex dendritic arbors, as a tractable model for conducting high-throughput RNAi screens aimed at identifying key regulators of dendritic branch formation. By carrying out two separate RNAi screens, a small-scale candidate-based screen and a large-scale screen of the ~3000 genes on chromosome IV, we retrieved 11 genes that either promote or suppress the formation of PVD-associated dendrites. We present a detailed functional characterization of one of the genes, bicd-1, which encodes a microtubule-associated protein previously shown to modulate the transport of mRNAs and organelles in a variety of organisms. Specifically, we describe a novel role for bicd-1 in regulating dendrite branch formation and show that bicd-1 is likely to be expressed, and primarily required, in PVD neurons to control dendritic branching. We also present evidence that bicd-1 operates in a conserved pathway with dhc-1 and unc-116, components of the dynein minus-end-directed and kinesin-1 plus-end-directed microtubule-based motor complexes, respectively, and interacts genetically with the repulsive guidance receptor unc-5.

Links

PubMed PMC3014636 Online version:10.1242/dev.060939

Keywords

Animals; Caenorhabditis elegans/cytology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cytoplasmic Dyneins/genetics; Cytoplasmic Dyneins/metabolism; Dendrites/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Kinesin/genetics; Kinesin/metabolism; Protein Binding; RNA Interference; Sensory Receptor Cells/cytology; Sensory Receptor Cells/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:E2JKZ0

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:G5ED34

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:BICD

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00093833

P

  • occurs_in:(WBbt:0006831)|occurs_in:(WBbt:0006828)

Seeded From UniProt

complete

CAEEL:BICD

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00006840

P

Seeded From UniProt

complete

CAEEL:BICD

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000962

P

Seeded From UniProt

complete

CAEEL:BICD

located_in

GO:0030425: dendrite

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:BICD

located_in

GO:0043025: neuronal cell body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:DYHC

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:DYHC

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00016611

P

Seeded From UniProt

complete

CAEEL:KINH

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00016611

P

Seeded From UniProt

complete

CAEEL:KINH

involved_in

GO:0048814: regulation of dendrite morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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