GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:21124943

From GONUTS
Jump to: navigation, search
Citation

Demarco, RS and Lundquist, EA (2010) RACK-1 acts with Rac GTPase signaling and UNC-115/abLIM in Caenorhabditis elegans axon pathfinding and cell migration. PLoS Genet. 6:e1001215

Abstract

Migrating cells and growth cones extend lamellipodial and filopodial protrusions that are required for outgrowth and guidance. The mechanisms of cytoskeletal regulation that underlie cell and growth cone migration are of much interest to developmental biologists. Previous studies have shown that the Arp2/3 complex and UNC-115/abLIM act redundantly to mediate growth cone lamellipodia and filopodia formation and axon pathfinding. While much is known about the regulation of Arp2/3, less is known about regulators of UNC-115/abLIM. Here we show that the Caenorhabditis elegans counterpart of the Receptor for Activated C Kinase (RACK-1) interacts physically with the actin-binding protein UNC-115/abLIM and that RACK-1 is required for axon pathfinding. Genetic interactions indicate that RACK-1 acts cell-autonomously in the UNC-115/abLIM pathway in axon pathfinding and lamellipodia and filopodia formation, downstream of the CED-10/Rac GTPase and in parallel to MIG-2/RhoG. Furthermore, we show that RACK-1 is involved in migration of the gonadal distal tip cells and that the signaling pathways involved in this process might be distinct from those involved in axon pathfinding. In sum, these studies pinpoint RACK-1 as a component of a novel signaling pathway involving Rac GTPases and UNC-115/abLIM and suggest that RACK-1 might be involved in the regulation of the actin cytoskeleton and lamellipodia and filopodia formation in migrating cells and growth cones.

Links

PubMed PMC2987834 Online version:10.1371/journal.pgen.1001215

Keywords

Amino Acid Sequence; Animals; Axons/metabolism; Base Sequence; Caenorhabditis elegans/cytology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Cell Movement; Green Fluorescent Proteins/metabolism; Immunoprecipitation; Microfilament Proteins/chemistry; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Models, Biological; Molecular Sequence Data; Motor Neurons/cytology; Motor Neurons/metabolism; Mutation/genetics; Organ Specificity; Protein Binding; Pseudopodia/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; Recombinant Fusion Proteins/metabolism; Signal Transduction; Two-Hybrid System Techniques; rac GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5EBV3

involved_in

GO:0008045: motor neuron axon guidance

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00010556

P

Seeded From UniProt

complete

CAEEL:G5EG53

involved_in

GO:0008045: motor neuron axon guidance

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00010556

P

Seeded From UniProt

complete

CAEEL:H2L0C7

involved_in

GO:0008045: motor neuron axon guidance

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00010556

P

Seeded From UniProt

complete

CAEEL:Q19240

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00010556

F

Seeded From UniProt

complete

CAEEL:GBLP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q95QM5

F

Seeded From UniProt

complete

CAEEL:GBLP

part_of

GO:0030424: axon

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GBLP

part_of

GO:0044297: cell body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GBLP

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GBLP

part_of

GO:0030426: growth cone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GBLP

involved_in

GO:0030334: regulation of cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251176

P

Seeded From UniProt

complete

CAEEL:GBLP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00006839

F

Seeded From UniProt

complete

CAEEL:GBLP

involved_in

GO:0008045: motor neuron axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00251176

P

Seeded From UniProt

complete

CAEEL:GBLP

involved_in

GO:0008045: motor neuron axon guidance

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00003239

P

Seeded From UniProt

complete

CAEEL:GBLP

involved_in

GO:0008045: motor neuron axon guidance

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00006770

P

Seeded From UniProt

complete

CAEEL:Q95QM5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q21215

F

Seeded From UniProt

complete

CAEEL:Q95QM5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00010556

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.