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PMID:21081150

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Citation

Polzien, L, Baljuls, A, Roth, HM, Kuper, J, Benz, R, Schweimer, K, Hekman, M and Rapp, UR (2011) Pore-forming activity of BAD is regulated by specific phosphorylation and structural transitions of the C-terminal part. Biochim. Biophys. Acta 1810:162-9

Abstract

BAD protein (Bcl-2 antagonist of cell death) belongs to the BH3-only subfamily of proapoptotic proteins and is proposed to function as the sentinel of the cellular health status. Physiological activity of BAD is regulated by phosphorylation, association with 14-3-3 proteins, binding to membrane lipids and pore formation. Since the functional role of the BAD C-terminal part has not been considered so far, we have investigated here the interplay of the structure and function of this region.

Links

PubMed Online version:10.1016/j.bbagen.2010.11.002

Keywords

14-3-3 Proteins/chemistry; 14-3-3 Proteins/genetics; 14-3-3 Proteins/metabolism; Amino Acid Sequence; Circular Dichroism; Glutathione Transferase/genetics; Glutathione Transferase/metabolism; Humans; Lipid Bilayers/chemistry; Lipid Bilayers/metabolism; Molecular Sequence Data; Peptides/chemistry; Peptides/metabolism; Phosphorylation; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Surface Plasmon Resonance; Water/chemistry; bcl-Associated Death Protein/chemistry; bcl-Associated Death Protein/genetics; bcl-Associated Death Protein/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:1433Z

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q92934

F

Seeded From UniProt

complete

HUMAN:B2CL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q92934

F

Seeded From UniProt

complete

HUMAN:BAD

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q07817

F

Seeded From UniProt

complete

HUMAN:BAD

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P63104

F

Seeded From UniProt

complete

HUMAN:Q6FH21

involved_in

GO:0046931: pore complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:Q6FH21

enables

GO:0005543: phospholipid binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:BAD

involved_in

GO:0046931: pore complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BAD

enables

GO:0005543: phospholipid binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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