PMID:21076391

Citation	Silhankova, M, Port, F, Harterink, M, Basler, K and Korswagen, HC (2010) Wnt signalling requires MTM-6 and MTM-9 myotubularin lipid-phosphatase function in Wnt-producing cells. EMBO J. 29:4094-105
Abstract	Wnt proteins are lipid-modified glycoproteins that have important roles in development, adult tissue homeostasis and disease. Secretion of Wnt proteins from producing cells is mediated by the Wnt-binding protein MIG-14/Wls, which binds Wnt in the Golgi network and transports it to the cell surface for release. It has recently been shown that recycling of MIG-14/Wls from the plasma membrane to the trans-Golgi network is required for efficient Wnt secretion, but the mechanism of this retrograde transport pathway is still poorly understood. In this study, we report the identification of MTM-6 and MTM-9 as novel regulators of MIG-14/Wls trafficking in Caenorhabditis elegans. MTM-6 and MTM-9 are myotubularin lipid phosphatases that function as a complex to dephosphorylate phosphatidylinositol-3-phosphate, a central regulator of endosomal trafficking. We show that mutation of mtm-6 or mtm-9 leads to defects in several Wnt-dependent processes and demonstrate that MTM-6 is required in Wnt-producing cells as part of the MIG-14/Wls-recycling pathway. This function is evolutionarily conserved, as the MTM-6 orthologue DMtm6 is required for Wls stability and Wg secretion in Drosophila. We conclude that regulation of endosomal trafficking by the MTM-6/MTM-9 myotubularin complex is required for the retromer-dependent recycling of MIG-14/Wls and Wnt secretion.
Links	PubMed PMC3018790 Online version:10.1038/emboj.2010.278
Keywords	Animals; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/genetics; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Carrier Proteins/metabolism; Drosophila/enzymology; Gene Knockdown Techniques; Gene Knockout Techniques; Phosphatidylinositol Phosphates/metabolism; Protein Tyrosine Phosphatases/genetics; Protein Tyrosine Phosphatases/metabolism; Protein Tyrosine Phosphatases, Non-Receptor/genetics; Protein Tyrosine Phosphatases, Non-Receptor/metabolism; Wnt Proteins/secretion
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAEEL:G5EBF2	involved_in	GO:0030334: regulation of cell migration	ECO:0000315: mutant phenotype evidence used in manual assertion	WB:WBVar00000266 WB:WBVar00000267 WB:WBVar00091628	P		Seeded From UniProt	complete
CAEEL:G5EBF2	involved_in	GO:0030111: regulation of Wnt signaling pathway	ECO:0000315: mutant phenotype evidence used in manual assertion	WB:WBVar00091628	P		Seeded From UniProt	complete
CAEEL:MTMR6	involved_in	GO:0030334: regulation of cell migration	ECO:0000315: mutant phenotype evidence used in manual assertion	WB:WBVar00000266 WB:WBVar00000267 WB:WBVar00091628	P		Seeded From UniProt	complete
CAEEL:MTMR6	involved_in	GO:0030111: regulation of Wnt signaling pathway	ECO:0000315: mutant phenotype evidence used in manual assertion	WB:WBVar00091628	P		Seeded From UniProt	complete
CAEEL:MTMR9	involved_in	GO:0030334: regulation of cell migration	ECO:0000315: mutant phenotype evidence used in manual assertion	WB:WBVar00000254	P		Seeded From UniProt	complete
CAEEL:Q9XW41	part_of	GO:0005768: endosome	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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