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PMID:21074047

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Citation

Harel-Sharvit, L, Eldad, N, Haimovich, G, Barkai, O, Duek, L and Choder, M (2010) RNA polymerase II subunits link transcription and mRNA decay to translation. Cell 143:552-63

Abstract

Little is known about crosstalk between the eukaryotic transcription and translation machineries that operate in different cell compartments. The yeast proteins Rpb4p and Rpb7p represent one such link as they form a heterodimer that shuttles between the nucleus, where it functions in transcription, and the cytoplasm, where it functions in the major mRNA decay pathways. Here we show that the Rpb4/7 heterodimer interacts physically and functionally with components of the translation initiation factor 3 (eIF3), and is required for efficient translation initiation. Efficient translation in the cytoplasm depends on association of Rpb4/7 with RNA polymerase II (Pol II) in the nucleus, leading to a model in which Pol II remotely controls translation. Hence, like in prokaryotes, the eukaryotic translation is coupled to transcription. We propose that Rpb4/7, through its interactions at each step in the mRNA lifecycle, represents a class of factors, "mRNA coordinators," which integrate the various stages of gene expression into a system.

Links

PubMed Online version:10.1016/j.cell.2010.10.033

Keywords

DNA-Directed RNA Polymerases/metabolism; Eukaryotic Initiation Factor-3/metabolism; Gene Expression Regulation, Fungal; Mutation; Protein Biosynthesis; RNA Polymerase II/metabolism; RNA Stability; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:RPB4

enables

GO:0031369: translation initiation factor binding

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000004926

F

Seeded From UniProt

complete

YEAST:RPB4

involved_in

GO:0045948: positive regulation of translational initiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:RPB4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P32497

F

Seeded From UniProt

complete

YEAST:RPB4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P34087

F

Seeded From UniProt

complete

YEAST:EIF3C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P20433

F

Seeded From UniProt

complete

YEAST:RPB7

enables

GO:0031369: translation initiation factor binding

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000004926

F

Seeded From UniProt

complete

YEAST:RPB7

involved_in

GO:0045948: positive regulation of translational initiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:RPB7

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P20433

F

Seeded From UniProt

complete


See also

References

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