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PMID:21045165

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Citation

Zhou, W, Wei, L, Xu, J, Zhai, Q, Jiang, H, Chen, R, Chen, Q, Sun, J, Chu, J, Zhu, L, Liu, CM and Li, C (2010) Arabidopsis Tyrosylprotein sulfotransferase acts in the auxin/PLETHORA pathway in regulating postembryonic maintenance of the root stem cell niche. Plant Cell 22:3692-709

Abstract

Recent identification of the Arabidopsis thaliana tyrosylprotein sulfotransferase (TPST) and a group of Tyr-sulfated peptides known as root meristem growth factors (RGFs) highlights the importance of protein Tyr sulfation in plant growth and development. Here, we report the action mechanism of TPST in maintenance of the root stem cell niche, which in the Arabidopsis root meristem is an area of four mitotically inactive quiescent cells plus the surrounding mitotically active stem cells. Mutation of TPST leads to defective maintenance of the root stem cell niche, decreased meristematic activity, and stunted root growth. We show that TPST expression is positively regulated by auxin and that mutation of this gene affects auxin distribution by reducing local expression levels of several PIN genes and auxin biosynthetic genes in the stem cell niche region. We also show that mutation of TPST impairs basal- and auxin-induced expression of the PLETHORA (PLT) stem cell transcription factor genes and that overexpression of PLT2 rescues the root meristem defects of the loss-of-function mutant of TPST. Together, these results support that TPST acts to maintain root stem cell niche by regulating basal- and auxin-induced expression of PLT1 and PLT2. TPST-dependent sulfation of RGFs provides a link between auxin and PLTs in regulating root stem cell niche maintenance.

Links

PubMed PMC3015123 Online version:10.1105/tpc.110.075721

Keywords

Arabidopsis/anatomy & histology; Arabidopsis/enzymology; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Gene Expression Regulation, Plant; Indoleacetic Acids/metabolism; Meristem/anatomy & histology; Meristem/growth & development; Meristem/metabolism; Mutation; Phylogeny; Plant Roots/cytology; Plant Roots/growth & development; Plant Roots/metabolism; Signal Transduction/physiology; Stem Cell Niche; Sulfotransferases/classification; Sulfotransferases/genetics; Sulfotransferases/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TPST

acts_upstream_of_or_within

GO:0009733: response to auxin

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPST

acts_upstream_of_or_within

GO:0019827: stem cell population maintenance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPST

acts_upstream_of_or_within

GO:0010082: regulation of root meristem growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TPST

acts_upstream_of_or_within

GO:0010468: regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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