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PMID:20980617

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Citation

Yasutis, K, Vignali, M, Ryder, M, Tameire, F, Dighe, SA, Fields, S and Kozminski, KG (2010) Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain. Mol. Biol. Cell 21:4373-86

Abstract

Deletion of the paralogs ZDS1 and ZDS2 in the budding yeast Saccharomyces cerevisiae causes a mis-regulation of polarized cell growth. Here we show a function for these genes as regulators of the Swe1p (Wee1p) kinase-dependent G2/M checkpoint. We identified a conserved domain in the C-terminus of Zds2p consisting of amino acids 813-912 (hereafter referred to as ZH4 for Zds homology 4) that is required for regulation of Swe1p-dependent polarized bud growth. ZH4 is shown by protein affinity assays to be necessary and sufficient for interaction with Cdc55p, a regulatory subunit of protein phosphatase 2A (PP2A). We hypothesized that the Zds proteins are in a pathway that negatively regulates the Swe1p-dependent G2/M checkpoint via Cdc55p. Supporting this model, deletion of CDC55 rescues the aberrant bud morphology of a zds1Δzds2Δ strain. We also show that expression of ZDS1 or ZDS2 from a strong galactose-inducible promoter can induce mitosis even when the Swe1p-dependent G2/M checkpoint is activated by mis-organization of the actin cytoskeleton. This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint.

Links

PubMed PMC3002390 Online version:10.1091/mbc.E10-04-0326

Keywords

Adaptor Proteins, Signal Transducing/genetics; Adaptor Proteins, Signal Transducing/metabolism; Amino Acid Sequence; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Conserved Sequence; G2 Phase; Gene Deletion; Mitosis; Mutation, Missense; Phosphorylation; Protein Interaction Mapping; Protein Phosphatase 2/genetics; Protein Phosphatase 2/metabolism; Protein-Tyrosine Kinases/genetics; Protein-Tyrosine Kinases/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Temperature; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ZDS1

involved_in

GO:0010971: positive regulation of G2/M transition of mitotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000003158
SGD:S000004577

P

Seeded From UniProt

complete

YEAST:ZDS2

involved_in

GO:0010971: positive regulation of G2/M transition of mitotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000003158
SGD:S000004886

P

Seeded From UniProt

complete

YEAST:ZDS2

located_in

GO:0005934: cellular bud tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:2ABA

involved_in

GO:0044818: mitotic G2/M transition checkpoint

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004577
SGD:S000004886

P

Seeded From UniProt

complete


See also

References

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