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PMID:20966047

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Citation

Saito, K, Ishizu, H, Komai, M, Kotani, H, Kawamura, Y, Nishida, KM, Siomi, H and Siomi, MC (2010) Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. Genes Dev. 24:2493-8

Abstract

PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.

Links

PubMed PMC2975925 Online version:10.1101/gad.1989510

Keywords

Animals; Cell Line; Cell Nucleus/metabolism; Cytoplasm/metabolism; DNA Transposable Elements/physiology; Drosophila Proteins/metabolism; Drosophila melanogaster/metabolism; Drosophila melanogaster/physiology; Endoribonucleases/metabolism; Protein Transport; RNA Helicases/metabolism; RNA, Small Interfering/biosynthesis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:ARMI

located_in

GO:0070725: Yb body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ARMI

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ZUC

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ZUC

NOT|located_in

GO:0043186: P granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ZUC

involved_in

GO:0034587: piRNA metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9W4W2

located_in

GO:0070725: Yb body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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