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PMID:20951776

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Citation

Guo, Y, Ma, L, Cristofanilli, M, Hart, RP, Hao, A and Schachner, M (2011) Transcription factor Sox11b is involved in spinal cord regeneration in adult zebrafish. Neuroscience 172:329-41

Abstract

Adult zebrafish have the ability to recover from spinal cord injury and exhibit re-growth of descending axons from the brainstem to the spinal cord. We performed gene expression analysis using microarray to find damage-induced genes after spinal cord injury, and found that Sox11b mRNA is up-regulated at 11 days after injury. However, the functional relevance of Sox11b for regeneration is not known. Here, we report that the up-regulation of Sox11b mRNA after spinal cord injury is mainly localized in ependymal cells lining the central canal and in newly differentiating neuronal precursors or immature neurons. Using an in vivo morpholino-based gene knockout approach, we demonstrate that Sox11b is essential for locomotor recovery after spinal cord injury. In the injured spinal cord, expression of the neural stem cell associated gene Nestin, and the proneural gene Ascl1a (Mash1a), which are involved in the self-renewal and cell fate specification of endogenous neural stem cells, respectively, is regulated by Sox11b. Our data indicate that Sox11b promotes neuronal determination of endogenous stem cells and regenerative neurogenesis following spinal cord injury in the adult zebrafish. Enhancing Sox11b expression to promote proliferation and neurogenic determination of endogenous neural stem cells after injury may be a promising strategy in restorative therapy after spinal cord injury in mammals.

Links

PubMed PMC3292217 Online version:10.1016/j.neuroscience.2010.10.026

Keywords

Animals; Disease Models, Animal; Neurogenesis/genetics; Recovery of Function/genetics; Regeneration/genetics; SOX Transcription Factors/genetics; SOX Transcription Factors/physiology; Spinal Cord/metabolism; Spinal Cord/pathology; Spinal Cord/physiopathology; Spinal Cord Injuries/metabolism; Spinal Cord Injuries/pathology; Spinal Cord Injuries/physiopathology; Zebrafish; Zebrafish Proteins/genetics; Zebrafish Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:Q5TZG4

involved_in

GO:0030182: neuron differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q5TZG4

involved_in

GO:0009611: response to wounding

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q7ZU05

involved_in

GO:0030182: neuron differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q7ZU05

involved_in

GO:0009611: response to wounding

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q9I9C7

involved_in

GO:0030182: neuron differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q9I9C7

involved_in

GO:0009611: response to wounding

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q9YH22

involved_in

GO:0030182: neuron differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete

DANRE:Q9YH22

involved_in

GO:0009611: response to wounding

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-110113-1

P

Seeded From UniProt

complete


See also

References

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