GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20951351

From GONUTS
Jump to: navigation, search
Citation

Matson, CK, Murphy, MW, Griswold, MD, Yoshida, S, Bardwell, VJ and Zarkower, D (2010) The mammalian doublesex homolog DMRT1 is a transcriptional gatekeeper that controls the mitosis versus meiosis decision in male germ cells. Dev. Cell 19:612-24

Abstract

The switch from mitosis to meiosis is a unique feature of germ cell development. In mammals, meiotic initiation requires retinoic acid (RA), which activates meiotic inducers, including Stra8, but how the switch to meiosis is controlled in male germ cells (spermatogonia) remains poorly understood. Here we examine the role of the Doublesex-related transcription factor DMRT1 in adult spermatogenesis using conditional gene targeting in the mouse. Loss of Dmrt1 causes spermatogonia to precociously exit the spermatogonial program and enter meiosis. Therefore, DMRT1 determines whether male germ cells undergo mitosis and spermatogonial differentiation or meiosis. Loss of Dmrt1 in spermatogonia also disrupts cyclical gene expression in Sertoli cells. DMRT1 acts in spermatogonia to restrict RA responsiveness, directly repress Stra8 transcription, and activate transcription of the spermatogonial differentiation factor Sohlh1, thereby preventing meiosis and promoting spermatogonial development. By coordinating spermatogonial development and mitotic amplification with meiosis, DMRT1 allows abundant, continuous production of sperm.

Links

PubMed PMC2996490 Online version:10.1016/j.devcel.2010.09.010

Keywords

Animals; Cell Differentiation/drug effects; Cell Proliferation/drug effects; DNA-Binding Proteins/chemistry; Drosophila Proteins/chemistry; Gene Deletion; Gene Expression Regulation, Developmental/drug effects; Kinetics; Male; Meiosis/drug effects; Meiosis/genetics; Mice; Mitosis/drug effects; Mitosis/genetics; Mutation/genetics; Promoter Regions, Genetic/genetics; Protein Binding/drug effects; Proteins/genetics; Sequence Homology, Amino Acid; Sertoli Cells/cytology; Sertoli Cells/drug effects; Sertoli Cells/metabolism; Spermatogenesis/drug effects; Spermatogenesis/genetics; Spermatogonia/cytology; Spermatogonia/drug effects; Spermatogonia/metabolism; Spermatozoa/cytology; Spermatozoa/drug effects; Spermatozoa/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic/drug effects; Tretinoin/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:DMRT1

involved_in

GO:0030238: male sex determination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DMRT1

involved_in

GO:0002176: male germ cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DMRT1

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DMRT1

enables

GO:0000987: cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:DMRT1

involved_in

GO:0046661: male sex differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DMRT1

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DMRT1

involved_in

GO:0045840: positive regulation of mitotic nuclear division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DMRT1

involved_in

GO:0045835: negative regulation of meiotic nuclear division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.