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PMID:20944413
| Citation |
Morohoshi, T, Fukamachi, K, Kato, M, Kato, N and Ikeda, T (2010) Regulation of the violacein biosynthetic gene cluster by acylhomoserine lactone-mediated quorum sensing in Chromobacterium violaceum ATCC 12472. Biosci. Biotechnol. Biochem. 74:2116-9 |
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| Abstract |
Chromobacterium violaceum produces the purple pigment violacein by quorum-sensing regulation. 20-bp of the lux box-like sequence was found upstream of vioA in C. violaceum ATCC 12472. CviR received C10-HSL and C6-HSL and activated the transcription of vioA in Escherichia coli. However, in strain ATCC 12472, C6-HSL inhibited both C10-HSL-mediated violacein production and the transcription of vioA. |
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| Keywords |
Acyl-Butyrolactones/pharmacology; Base Sequence; Chromobacterium/cytology; Chromobacterium/drug effects; Chromobacterium/genetics; Chromobacterium/metabolism; Dose-Response Relationship, Drug; Genes, Bacterial/genetics; Indoles/metabolism; Molecular Sequence Data; Multigene Family/genetics; Quorum Sensing/drug effects; Transcription, Genetic/drug effects |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009370 : quorum sensing response regulator activity |
ECO:0000315: |
1 |
Fig. 2. effects of C10-HSL and C6-HSL on Violacein Production and Transcription of vioA in Strain VIR20 and E. coli DH5alpha. VIR20 harboring pJN105AZ and E. coli DH5alpha harboring pQF50CA and pJN105CR were grown in LB medium with various concentration of C10-HSL (solid squares) and C6-HSL (solid circles). After 15 h of incubation, Beta-galactosidase activity in VIR20 (A), violacein production in VIR20 (B), and Beta-galactosidase activity in E. coli DH5alpha (C) were measured. A competition assay was performed at a range of concentrations of C6-HSL and 100 nM C10-HSL (open squares). The results were reproduced in three repeated experiments. Error bars indicate standard deviation. |
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