PMID:20938646

Citation	Xu, L, Wang, Q, Xiao, J, Liu, Q, Wang, X, Chen, T and Zhang, Y (2010) Characterization of Edwardsiella tarda waaL: roles in lipopolysaccharide biosynthesis, stress adaptation, and virulence toward fish. Arch. Microbiol. 192:1039-47
Abstract	Edwardsiella tarda is the causative agent of edwardsiellosis in fish. The genome sequence of a virulent strain EIB202 has been determined. According to the genome sequence, the lipopolysaccharide (LPS) synthesis cluster containing a putative O-antigen ligase gene waaL was identified. Here, the in-frame deletion mutant ΔwaaL was constructed to analyze the function of WaaL in E. tarda EIB202. The ΔwaaL mutant displayed absence in O-antigen side chains in the LPS production. The ΔwaaL mutant exhibited an increased sensitivity to hydrogen peroxide indicating that the LPS was involved in the endurance to the oxidative stress in hosts during infection. In addition, the resistance of ΔwaaL to serum and polymyxin B decreased remarkably. The ΔwaaL mutant was also attenuated in virulence, showed an impaired ability in internalization of epithelioma papulosum cyprinid (EPC) cells and a comparatively poor ability of proliferation in vivo, which was in line with the increased LD(50) value. These results indicated that waaL gene was a functional member of the gene cluster involved in LPS synthesis and highlighted the importance of the O-antigen side chains to stress adaption and virulence in E. tarda, signifying the gene as a potential target for live attenuated vaccine against this bacterium.
Links	PubMed Online version:10.1007/s00203-010-0635-z
Keywords	Adaptation, Physiological; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carbon-Oxygen Ligases/genetics; Carbon-Oxygen Ligases/metabolism; Cells, Cultured; DNA, Bacterial/genetics; Edwardsiella tarda/enzymology; Edwardsiella tarda/genetics; Edwardsiella tarda/pathogenicity; Enterobacteriaceae Infections/microbiology; Fish Diseases/microbiology; Genes, Bacterial; Lipopolysaccharides/biosynthesis; Multigene Family; Mutation; O Antigens/chemistry; Oxidative Stress; Stress, Physiological; Virulence; Zebrafish/microbiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EDWTE:D0Z953	involved_in	GO:0009103: lipopolysaccharide biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
EDWTE:D0Z953		GO:0009103: lipopolysaccharide biosynthetic process	ECO:0000315:		P		Figure 2C confirmed that WaaL was involved in the LPS biosynthesis in Edwardsiella tarda.	complete CACAO 1973

References

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