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PMID:20937886

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Citation

Guo, L, Yu, Y, Law, JA and Zhang, X (2010) SET DOMAIN GROUP2 is the major histone H3 lysine [corrected] 4 trimethyltransferase in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 107:18557-62

Abstract

Posttranslational modifications of histones play important roles in modulating chromatin structure and regulating gene expression. We have previously shown that more than two thirds of Arabidopsis genes contain histone H3 methylation at lysine 4 (H3K4me) and that trimethylation of H3K4 (H3K4me3) is preferentially located at actively transcribed genes. In addition, several Arabidopsis mutants with locus-specific loss of H3K4me have been found to display various developmental abnormalities. These findings suggest that H3K4me3 may play important roles in maintaining the normal expression of a large number of genes. However, the major enzyme(s) responsible for H3K4me3 has yet to be identified in plants, making it difficult to address questions regarding the mechanisms and functions of H3K4me3. Here we described the characterization of SET DOMAIN GROUP 2 (SDG2), a large Arabidopsis protein containing a histone lysine methyltransferase domain. We found that SDG2 homologs are highly conserved in plants and that the Arabidopsis SDG2 gene is broadly expressed during development. In addition, the loss of SDG2 leads to severe and pleiotropic phenotypes, as well as the misregulation of a large number of genes. Consistent with our finding that SDG2 is a robust and specific H3K4 methyltransferase in vitro, the loss of SDG2 leads to a drastic decrease in H3K4me3 in vivo. Taken together, these results suggest that SDG2 is the major enzyme responsible for H3K4me3 in Arabidopsis and that SDG2-dependent H3K4m3 is critical for regulating gene expression and plant development.

Links

PubMed PMC2972934 Online version:10.1073/pnas.1010478107

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Conserved Sequence; DNA Primers/genetics; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes, Plant; Histone-Lysine N-Methyltransferase/genetics; Histone-Lysine N-Methyltransferase/metabolism; Histones/chemistry; Histones/metabolism; Mutation; Phylogeny; Plants, Genetically Modified

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ATXR3

acts_upstream_of_or_within

GO:0010228: vegetative to reproductive phase transition of meristem

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATXR3

acts_upstream_of_or_within

GO:0009791: post-embryonic development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATXR3

enables

GO:0042800: histone methyltransferase activity (H3-K4 specific)

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ATXR3

acts_upstream_of_or_within

GO:0048443: stamen development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATXR3

acts_upstream_of_or_within

GO:0048440: carpel development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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