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PMID:20876334

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Citation

Yokotani, N, Higuchi, M, Kondou, Y, Ichikawa, T, Iwabuchi, M, Hirochika, H, Matsui, M and Oda, K (2011) A novel chloroplast protein, CEST induces tolerance to multiple environmental stresses and reduces photooxidative damage in transgenic Arabidopsis. J. Exp. Bot. 62:557-69

Abstract

Environmental stresses are major factors in limiting plant growth and crop production. To find genes improving salt tolerance, the screening of a large population of transgenic Arabidopsis thaliana that expressed rice full-length cDNAs under salinity stress is reported here. In this study one of the isolated salt-tolerant lines, R07303 was analysed in detail. An uncharacterized rice gene CHLOROPLAST PROTEIN-ENHANCING STRESS TOLERANCE (OsCEST) was integrated in R07303. Newly constructed transgenic Arabidopsis that overexpressed OsCEST or its Arabidopsis homologue AtCEST showed improved tolerance to salinity stress. OsCEST and AtCEST were mainly transcribed in photosynthetic tissues. Green fluorescent protein-fused OsCEST and AtCEST proteins were localized to the chloroplast in the Arabidopsis leaf protoplasts. CEST-overexpressing Arabidopsis showed enhanced tolerance not only to salt stress but also to drought stress, high-temperature stress, and paraquat, which causes photooxidative stress. Under saline conditions, overexpression of CESTs modulated the stress-induced impairment of photosynthetic activity and the peroxidation of lipids. Reduced expression of AtCEST because of double-stranded RNA interference resulted in the impairment of photosynthetic activity, the reduction of green pigment, defects in chloroplast development, and growth retardation under light. This paper discusses the relationship between the chloroplast protein CEST and photooxidative damage.

Links

PubMed Online version:10.1093/jxb/erq290

Keywords

Amino Acid Sequence; Arabidopsis/chemistry; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis/radiation effects; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Chloroplasts/chemistry; Chloroplasts/genetics; Chloroplasts/metabolism; Gene Expression Regulation, Plant/radiation effects; Hot Temperature; Light; Molecular Sequence Data; Oryza sativa/genetics; Oryza sativa/metabolism; Oxidative Stress/radiation effects; Photosynthesis; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified/chemistry; Plants, Genetically Modified/genetics; Plants, Genetically Modified/physiology; Plants, Genetically Modified/radiation effects; Salt-Tolerance; Sequence Alignment; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ORYSJ:CEST

involved_in

GO:0010286: heat acclimation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:CEST

located_in

GO:0009535: chloroplast thylakoid membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ORYSJ:CEST

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:CEST

involved_in

GO:0009414: response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:CEST

involved_in

GO:0080183: response to photooxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:CEST

involved_in

GO:0042538: hyperosmotic salinity response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Y3IP1

involved_in

GO:0010286: heat acclimation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Y3IP1

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:Y3IP1

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Y3IP1

involved_in

GO:0009414: response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Y3IP1

involved_in

GO:0080183: response to photooxidative stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Y3IP1

involved_in

GO:0042538: hyperosmotic salinity response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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