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PMID:2087220

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Citation

Meyer, RR and Laine, PS (1990) The single-stranded DNA-binding protein of Escherichia coli. Microbiol. Rev. 54:342-80

Abstract

The single-stranded DNA-binding protein (SSB) of Escherichia coli is involved in all aspects of DNA metabolism: replication, repair, and recombination. In solution, the protein exists as a homotetramer of 18,843-kilodalton subunits. As it binds tightly and cooperatively to single-stranded DNA, it has become a prototypic model protein for studying protein-nucleic acid interactions. The sequences of the gene and protein are known, and the functional domains of subunit interaction, DNA binding, and protein-protein interactions have been probed by structure-function analyses of various mutations. The ssb gene has three promoters, one of which is inducible because it lies only two nucleotides from the LexA-binding site of the adjacent uvrA gene. Induction of the SOS response, however, does not lead to significant increases in SSB levels. The binding protein has several functions in DNA replication, including enhancement of helix destabilization by DNA helicases, prevention of reannealing of the single strands and protection from nuclease digestion, organization and stabilization of replication origins, primosome assembly, priming specificity, enhancement of replication fidelity, enhancement of polymerase processivity, and promotion of polymerase binding to the template. E. coli SSB is required for methyl-directed mismatch repair, induction of the SOS response, and recombinational repair. During recombination, SSB interacts with the RecBCD enzyme to find Chi sites, promotes binding of RecA protein, and promotes strand uptake.

Links

PubMed PMC372786

Keywords

Amino Acid Sequence; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Molecular Sequence Data; Protein Conformation; Regulatory Sequences, Nucleic Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:SSB

acts_upstream_of_or_within

GO:0000725: recombinational repair

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SSB

acts_upstream_of_or_within

GO:0009432: SOS response

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SSB

acts_upstream_of_or_within

GO:0006298: mismatch repair

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SSB

involved_in

GO:0009432: SOS response

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SSB

involved_in

GO:0006298: mismatch repair

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SSB

involved_in

GO:0000725: recombinational repair

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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